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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12274 and 12275 are separated by 258 nucleotides
12275 and 12276 are separated by 366 nucleotides
12276 and 12277 are separated by 100 nucleotides
12277 and 12278 are separated by 790 nucleotides
mRNA_3906: 12274 - K03495 gidA, mnmG, MTO1 tRNA uridine 5-carboxymethylaminomethyl modification enzyme, at 865,746 to 868,815
12274
mRNA_3907: 12275 - Hypothetical Protein, at 869,074 to 869,751
12275
mRNA_3908: 12276 - Interpro Protein of unknown function (DUF2945), at 870,118 to 872,012
12276
mRNA_3909: 12277 - K10638 UHRF1, NP95 E3 ubiquitin-protein ligase UHRF1, at 872,113 to 873,418
12277
mRNA_3910: 12278 - KOG0587 Traf2- and Nck-interacting kinase and related germinal center kinase (GCK) family protein kinases, at 874,209 to 878,555
12278
Group
Condition
mRNA
_3906
mRNA
_3907
mRNA
_3908
mRNA
_3909
mRNA
_3910
12274
12275
12276
12277
12278
facs on lipid accumulation
Sorted High BODIPY Gate
-0.2
-1.9
-0.1
-0.1
+0.1
growth on oleic acid
YNB Oleic Acid
-0.3
-1.5
+0.0
+0.2
-0.1
growth on oleic acid
YNB Glucose
+0.1
-1.0
-0.1
+0.3
-0.3
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.5
-0.5
+0.0
+0.2
+0.1
facs on lipid accumulation
Sorted No BODIPY Gate
+0.4
-0.5
-0.0
-0.3
-0.2
auxotrophy
YNB Glucose 75mM Methionine
+0.3
-0.6
-0.1
+0.1
-0.4
auxotrophy
Dropout Complete
-0.1
-0.3
-0.1
+0.1
-0.2
auxotrophy
YNB Glucose 75mM Arginine
+0.1
-0.5
-0.1
+0.4
-0.2
growth on ricinoleic acid
YNB Glucose
-0.3
-0.4
+0.2
+0.0
+0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.3
-0.3
-0.1
-0.2
+0.2
auxotrophy
YNB Glucose
+0.1
-0.5
+0.1
+0.3
-0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.0
-0.7
+0.0
+0.6
-0.0
ypd buoyancy
40 hrs High Buoyancy
-0.1
-0.3
+0.1
+0.3
+0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.3
-0.9
+0.1
+0.7
+0.1
ypd buoyancy
40 hrs Low Buoyancy
-0.1
+0.2
+0.0
+0.1
+0.0
growth on ypd
YPD
-0.0
+0.2
-0.1
+0.7
-0.3
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.3
-1.0
+0.1
+0.9
+0.2
ypd buoyancy
40 hrs Median Buoyancy
+0.2
+0.1
+0.1
+0.2
+0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.4
+0.9
+0.2
+0.2
+0.2
ypd buoyancy
40 hrs No Buoyancy Separation
+0.5
+0.6
+0.1
+0.5
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.5
+0.7
+0.2
+0.7
+0.3
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