Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12277 and 12278 are separated by 790 nucleotides12278 and 12279 are separated by 327 nucleotides12279 and 12280 are separated by 147 nucleotides12280 and 12281 are separated by 209 nucleotides mRNA_3909: 12277 - K10638 UHRF1, NP95 E3 ubiquitin-protein ligase UHRF1, at 872,113 to 873,418 12277 mRNA_3910: 12278 - KOG0587 Traf2- and Nck-interacting kinase and related germinal center kinase (GCK) family protein kinases, at 874,209 to 878,555 12278 mRNA_3911: 12279 - K18655 DDX19, DBP5 ATP-dependent RNA helicase DDX19/DBP5, at 878,883 to 880,998 12279 mRNA_3912: 12280 - HMMPfam-Protein of unknown function (DUF2422)-PF10337, at 881,146 to 884,820 12280 mRNA_3913: 12281 - SUPERFAMILY--SSF48452, at 885,030 to 886,525 12281
Group Condition mRNA_3909 mRNA_3910 mRNA_3911 mRNA_3912 mRNA_3913
12277 12278 12279 12280 12281
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.4 N.D. -0.6 N.D.
auxotrophy Dropout Complete +0.1 -0.2 N.D. -0.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.2 N.D. -0.1 N.D.
auxotrophy YNB Glucose +0.3 -0.1 N.D. -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.1 N.D. -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.2 N.D. -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.2 N.D. -0.2 N.D.
growth on ypd YPD +0.7 -0.3 N.D. -0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 N.D. -0.0 N.D.
growth on ricinoleic acid YNB Glucose +0.0 +0.2 N.D. -0.1 N.D.
growth on oleic acid YNB Glucose +0.3 -0.3 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.0 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.1 N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.2 -0.1 N.D. +0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 +0.1 N.D. -0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.2 N.D. +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.2 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 +0.1 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 -0.0 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.9 +0.2 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 +0.3 N.D. +0.1 N.D.
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