Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12278 and 12279 are separated by 327 nucleotides12279 and 12280 are separated by 147 nucleotides12280 and 12281 are separated by 209 nucleotides12281 and 12282 are separated by 290 nucleotides mRNA_3910: 12278 - KOG0587 Traf2- and Nck-interacting kinase and related germinal center kinase (GCK) family protein kinases, at 874,209 to 878,555 12278 mRNA_3911: 12279 - K18655 DDX19, DBP5 ATP-dependent RNA helicase DDX19/DBP5, at 878,883 to 880,998 12279 mRNA_3912: 12280 - HMMPfam-Protein of unknown function (DUF2422)-PF10337, at 881,146 to 884,820 12280 mRNA_3913: 12281 - SUPERFAMILY--SSF48452, at 885,030 to 886,525 12281 mRNA_3914: 12282 - HMMPfam-Domain of unknown function (DUF1708)-PF08101,ProSiteProfiles-Rho GTPase-activating proteins domain profile.-PS50238,SMART-GTPase-activator protein for Rho-like GTPases-SM00324,SUPERFAMILY--SSF48350, at 886,816 to 890,417 12282
Group Condition mRNA_3910 mRNA_3911 mRNA_3912 mRNA_3913 mRNA_3914
12278 12279 12280 12281 12282
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. -0.6 N.D. -0.4
auxotrophy Dropout Complete -0.2 N.D. -0.6 N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.2 N.D. -0.4
auxotrophy YNB Glucose -0.1 N.D. -0.4 N.D. -0.3
growth on ypd YPD -0.3 N.D. -0.4 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.1 N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.1 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. +0.1 N.D. -0.1
growth on oleic acid YNB Glucose -0.3 N.D. +0.2 N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.1 N.D. -0.2
growth on ricinoleic acid YNB Glucose +0.2 N.D. -0.1 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. +0.2 N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. -0.0 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. +0.2 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. +0.1 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. -0.0 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.2 N.D. -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.0 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.1 N.D. +0.4 N.D. +0.0
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