Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12279 and 12280 are separated by 147 nucleotides12280 and 12281 are separated by 209 nucleotides12281 and 12282 are separated by 290 nucleotides12282 and 12283 are separated by 222 nucleotides mRNA_3911: 12279 - K18655 DDX19, DBP5 ATP-dependent RNA helicase DDX19/DBP5, at 878,883 to 880,998 12279 mRNA_3912: 12280 - HMMPfam-Protein of unknown function (DUF2422)-PF10337, at 881,146 to 884,820 12280 mRNA_3913: 12281 - SUPERFAMILY--SSF48452, at 885,030 to 886,525 12281 mRNA_3914: 12282 - HMMPfam-Domain of unknown function (DUF1708)-PF08101,ProSiteProfiles-Rho GTPase-activating proteins domain profile.-PS50238,SMART-GTPase-activator protein for Rho-like GTPases-SM00324,SUPERFAMILY--SSF48350, at 886,816 to 890,417 12282 mRNA_3915: 12283 - K01062 PLA2G7, PAFAH platelet-activating factor acetylhydrolase, at 890,640 to 892,402 12283
Group Condition mRNA_3911 mRNA_3912 mRNA_3913 mRNA_3914 mRNA_3915
12279 12280 12281 12282 12283
auxotrophy Dropout Complete N.D. -0.6 N.D. -0.5 -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. -0.6 N.D. -0.4 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 N.D. -0.2 -0.8
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 N.D. -0.0 -1.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. -0.2 -0.5
auxotrophy YNB Glucose N.D. -0.4 N.D. -0.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 N.D. -0.2 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 N.D. -0.4 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 N.D. -0.3 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -0.1 -0.3
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. -0.2 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 N.D. -0.2 +0.1
growth on oleic acid YNB Glucose N.D. +0.2 N.D. +0.0 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 N.D. -0.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 N.D. +0.1 -0.0
growth on oleic acid YNB Oleic Acid N.D. +0.4 N.D. +0.0 -0.2
growth on ypd YPD N.D. -0.4 N.D. +0.2 +0.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 N.D. +0.2 +0.7
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