Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12281 and 12282 are separated by 290 nucleotides12282 and 12283 are separated by 222 nucleotides12283 and 12284 are separated by 124 nucleotides12284 and 12285 are separated by 144 nucleotides mRNA_3913: 12281 - SUPERFAMILY--SSF48452, at 885,030 to 886,525 12281 mRNA_3914: 12282 - HMMPfam-Domain of unknown function (DUF1708)-PF08101,ProSiteProfiles-Rho GTPase-activating proteins domain profile.-PS50238,SMART-GTPase-activator protein for Rho-like GTPases-SM00324,SUPERFAMILY--SSF48350, at 886,816 to 890,417 12282 mRNA_3915: 12283 - K01062 PLA2G7, PAFAH platelet-activating factor acetylhydrolase, at 890,640 to 892,402 12283 mRNA_3916: 12284 - K02736 PSMB4 20S proteasome subunit beta 7, at 892,527 to 893,886 12284 mRNA_3917: 12285 - KOG4479 Transcription factor e(y)2, at 894,031 to 894,594 12285
Group Condition mRNA_3913 mRNA_3914 mRNA_3915 mRNA_3916 mRNA_3917
12281 12282 12283 12284 12285
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 -0.8 N.D. -0.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 +0.1 N.D. -1.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -0.4 N.D. -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 -1.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.3 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -0.5 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 -0.2 N.D. -0.0
growth on ricinoleic acid YNB Glucose N.D. -0.1 -0.1 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 +0.7 N.D. -1.0
growth on oleic acid YNB Oleic Acid N.D. +0.0 -0.2 N.D. +0.2
auxotrophy YNB Glucose N.D. -0.3 +0.1 N.D. +0.3
auxotrophy Dropout Complete N.D. -0.5 -0.4 N.D. +1.0
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 -0.3 N.D. +0.5
growth on oleic acid YNB Glucose N.D. +0.0 -0.4 N.D. +0.6
growth on ypd YPD N.D. +0.2 +0.7 N.D. -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.1 N.D. +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.1 N.D. +0.6
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 -0.2 N.D. +1.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.0 N.D. +0.7
auxotrophy YNB Glucose 75mM Arginine N.D. -0.4 +0.3 N.D. +1.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 -0.0 N.D. +1.0
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