Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12283 and 12284 are separated by 124 nucleotides12284 and 12285 are separated by 144 nucleotides12285 and 12286 are separated by 591 nucleotides12286 and 12287 are separated by 518 nucleotides mRNA_3915: 12283 - K01062 PLA2G7, PAFAH platelet-activating factor acetylhydrolase, at 890,640 to 892,402 12283 mRNA_3916: 12284 - K02736 PSMB4 20S proteasome subunit beta 7, at 892,527 to 893,886 12284 mRNA_3917: 12285 - KOG4479 Transcription factor e(y)2, at 894,031 to 894,594 12285 mRNA_3918: 12286 - KOG0024 Sorbitol dehydrogenase, at 895,186 to 897,368 12286 mRNA_3919: 12287 - KOG4020 Protein DRE2, required for cell viability, at 897,887 to 899,209 12287
Group Condition mRNA_3915 mRNA_3916 mRNA_3917 mRNA_3918 mRNA_3919
12283 12284 12285 12286 12287
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -1.3 -0.9 N.D.
ypd buoyancy 40 hrs High Buoyancy -1.0 N.D. +0.2 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. -0.1 -0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.8 N.D. -0.6 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. -0.6 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. +0.1 -0.1 N.D.
growth on ypd YPD +0.7 N.D. -0.6 -0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.0 +0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.2 +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 N.D. -1.0 +0.4 N.D.
growth on oleic acid YNB Glucose -0.4 N.D. +0.6 -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. +0.5 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. +0.6 -0.4 N.D.
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.2 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. +0.6 +0.1 N.D.
auxotrophy YNB Glucose +0.1 N.D. +0.3 +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.7 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. +1.0 +0.0 N.D.
auxotrophy Dropout Complete -0.4 N.D. +1.0 +0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. +1.2 +0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. +1.1 +0.4 N.D.
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