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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12283 and 12284 are separated by 124 nucleotides
12284 and 12285 are separated by 144 nucleotides
12285 and 12286 are separated by 591 nucleotides
12286 and 12287 are separated by 518 nucleotides
mRNA_3915: 12283 - K01062 PLA2G7, PAFAH platelet-activating factor acetylhydrolase, at 890,640 to 892,402
12283
mRNA_3916: 12284 - K02736 PSMB4 20S proteasome subunit beta 7, at 892,527 to 893,886
12284
mRNA_3917: 12285 - KOG4479 Transcription factor e(y)2, at 894,031 to 894,594
12285
mRNA_3918: 12286 - KOG0024 Sorbitol dehydrogenase, at 895,186 to 897,368
12286
mRNA_3919: 12287 - KOG4020 Protein DRE2, required for cell viability, at 897,887 to 899,209
12287
Group
Condition
mRNA
_3915
mRNA
_3916
mRNA
_3917
mRNA
_3918
mRNA
_3919
12283
12284
12285
12286
12287
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
N.D.
-1.3
-0.9
N.D.
ypd buoyancy
40 hrs High Buoyancy
-1.0
N.D.
+0.2
-0.4
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.3
N.D.
-0.1
-0.5
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.8
N.D.
-0.6
+0.3
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.4
N.D.
-0.6
+0.1
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
-0.5
N.D.
+0.1
-0.1
N.D.
growth on ypd
YPD
+0.7
N.D.
-0.6
-0.3
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.2
N.D.
-0.0
+0.1
N.D.
growth on ricinoleic acid
YNB Glucose
-0.1
N.D.
-0.2
+0.2
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
+0.7
N.D.
-1.0
+0.4
N.D.
growth on oleic acid
YNB Glucose
-0.4
N.D.
+0.6
-0.0
N.D.
ypd buoyancy
40 hrs Low Buoyancy
-0.3
N.D.
+0.5
+0.0
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
N.D.
+0.6
-0.4
N.D.
growth on oleic acid
YNB Oleic Acid
-0.2
N.D.
+0.2
+0.6
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.1
N.D.
+0.6
+0.1
N.D.
auxotrophy
YNB Glucose
+0.1
N.D.
+0.3
+0.3
N.D.
ypd buoyancy
40 hrs Median Buoyancy
-0.0
N.D.
+0.7
+0.1
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.0
N.D.
+1.0
+0.0
N.D.
auxotrophy
Dropout Complete
-0.4
N.D.
+1.0
+0.4
N.D.
auxotrophy
YNB Glucose 75mM Methionine
-0.2
N.D.
+1.2
+0.5
N.D.
auxotrophy
YNB Glucose 75mM Arginine
+0.3
N.D.
+1.1
+0.4
N.D.
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