Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12286 and 12287 are separated by 518 nucleotides12287 and 12288 are separated by 330 nucleotides12288 and 12289 are separated by 1294 nucleotides12289 and 12290 are separated by 442 nucleotides mRNA_3918: 12286 - KOG0024 Sorbitol dehydrogenase, at 895,186 to 897,368 12286 mRNA_3919: 12287 - KOG4020 Protein DRE2, required for cell viability, at 897,887 to 899,209 12287 mRNA_3920: 12288 - K12767 RIM15 serine/threonine-protein kinase RIM15, at 899,540 to 908,734 12288 mRNA_3921: 12289 - Hypothetical Protein, at 910,029 to 911,741 12289 mRNA_3922: 12290 - K11099 SNRPG, SMG small nuclear ribonucleoprotein G, at 912,184 to 912,720 12290
Group Condition mRNA_3918 mRNA_3919 mRNA_3920 mRNA_3921 mRNA_3922
12286 12287 12288 12289 12290
facs on lipid accumulation Sorted High BODIPY Gate -0.9 N.D. -1.7 +0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. -1.2 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. -0.1 -0.8 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -1.1 -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.4 N.D. +0.0 -0.8 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. +0.0 -0.6 N.D.
growth on ypd YPD -0.3 N.D. -0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. -0.1 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.4 +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.0 -0.3 N.D.
auxotrophy YNB Glucose +0.3 N.D. +0.3 -0.8 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. +0.1 -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. -0.2 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.5 N.D. +0.2 -0.6 N.D.
growth on ricinoleic acid YNB Glucose +0.2 N.D. +0.1 -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. +0.2 -0.2 N.D.
auxotrophy Dropout Complete +0.4 N.D. +0.3 -0.3 N.D.
growth on oleic acid YNB Glucose -0.0 N.D. +0.0 +0.7 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.2 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. +0.3 +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.6 N.D. +0.5 +0.5 N.D.
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