Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12287 and 12288 are separated by 330 nucleotides12288 and 12289 are separated by 1294 nucleotides12289 and 12290 are separated by 442 nucleotides12290 and 12291 are separated by 282 nucleotides mRNA_3919: 12287 - KOG4020 Protein DRE2, required for cell viability, at 897,887 to 899,209 12287 mRNA_3920: 12288 - K12767 RIM15 serine/threonine-protein kinase RIM15, at 899,540 to 908,734 12288 mRNA_3921: 12289 - Hypothetical Protein, at 910,029 to 911,741 12289 mRNA_3922: 12290 - K11099 SNRPG, SMG small nuclear ribonucleoprotein G, at 912,184 to 912,720 12290 mRNA_3923: 12291 - K01465 URA4, pyrC dihydroorotase, at 913,003 to 914,315 12291
Group Condition mRNA_3919 mRNA_3920 mRNA_3921 mRNA_3922 mRNA_3923
12287 12288 12289 12290 12291
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.7 +0.3 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.2 -0.2 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.1 -0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.8 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 -0.8 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 -0.6 N.D. N.D.
auxotrophy YNB Glucose N.D. +0.3 -0.8 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -0.6 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 +0.0 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.3 N.D. N.D.
growth on ypd YPD N.D. -0.1 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.1 N.D. N.D.
auxotrophy Dropout Complete N.D. +0.3 -0.3 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 +0.3 N.D. N.D.
growth on oleic acid YNB Glucose N.D. +0.0 +0.7 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.5 +0.5 N.D. N.D.
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