Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12294 and 12295 are separated by 157 nucleotides12295 and 12296 are separated by 225 nucleotides12296 and 12297 are separated by 211 nucleotides12297 and 12298 are separated by 366 nucleotides mRNA_3926: 12294 - HMMPfam-ER protein Pkr1-PF08636, at 919,864 to 921,279 12294 mRNA_3927: 12295 - K10734 GINS3 GINS complex subunit 3, at 921,437 to 922,417 12295 mRNA_3928: 12296 - HMMPfam-Fungalysin metallopeptidase (M36)-PF02128,HMMPfam-Fungalysin/Thermolysin Propeptide Motif-PF07504,PRINTS-Fungalysin metallopeptidase (M36) signature-PR00999,SUPERFAMILY--SSF55486, at 922,643 to 925,532 12296 mRNA_3929: 12297 - BLAST TKL/TKL-ccin protein kinase [Mycena chlorophos], at 925,744 to 929,341 12297 mRNA_3930: 12298 - Hypothetical Protein, at 929,708 to 931,043 12298
Group Condition mRNA_3926 mRNA_3927 mRNA_3928 mRNA_3929 mRNA_3930
12294 12295 12296 12297 12298
growth on ypd YPD -0.1 N.D. +0.0 -0.1 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.2 -0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. -0.1 -0.2 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.2 -0.0 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. +0.1 -0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. +0.0 -0.5 -0.2
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.0 -0.3 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. +0.1 -0.6 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.0 -0.1 -0.0
auxotrophy Dropout Complete +0.7 N.D. -0.2 +0.1 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. +0.2 -0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. -0.1 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.3 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.2 -0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. -0.2 -0.0 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.0 +0.1 -0.0
growth on oleic acid YNB Glucose +0.2 N.D. -0.1 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. +0.3 -0.1 +0.2
auxotrophy YNB Glucose +0.4 N.D. +0.0 +0.1 -0.1
growth on oleic acid YNB Oleic Acid +0.4 N.D. +0.2 -0.0 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. +0.5 -0.2 +0.7
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