Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12319 and 12320 are separated by 85 nucleotides12320 and 12321 are separated by 162 nucleotides12321 and 12322 are separated by 362 nucleotides12322 and 12323 are separated by 284 nucleotides mRNA_3951: 12319 - K02967 RP-S2, MRPS2, rpsB small subunit ribosomal protein S2, at 980,459 to 981,716 12319 mRNA_3952: 12320 - KOG1018 Cytosine deaminase FCY1 and related enzymes, at 981,802 to 983,195 12320 mRNA_3953: 12321 - Hypothetical Protein, at 983,358 to 984,692 12321 mRNA_3954: 12322 - KOG1426 FOG RCC1 domain, at 985,055 to 988,023 12322 mRNA_3955: 12323 - K02355 fusA, GFM, EFG elongation factor G, at 988,308 to 991,427 12323
Group Condition mRNA_3951 mRNA_3952 mRNA_3953 mRNA_3954 mRNA_3955
12319 12320 12321 12322 12323
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 +0.0 +0.2 -3.7
growth on ricinoleic acid YNB Glucose N.D. +0.3 -0.0 +0.3 -3.4
auxotrophy Dropout Complete N.D. +0.3 +0.1 +0.1 -3.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 -0.1 +0.1 -2.9
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.7 -0.6 -0.3 -0.3
auxotrophy YNB Glucose N.D. +0.2 +0.3 +0.1 -2.5
growth on oleic acid YNB Glucose N.D. -0.1 +0.3 +0.0 -2.0
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 -0.2 -0.3 -1.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 +0.2 +0.1 -2.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 -0.4 -0.2 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 -0.0 -0.1 -0.5
growth on oleic acid YNB Oleic Acid N.D. -0.0 +0.4 +0.2 -1.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.4 -0.0 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 -0.0 -0.3 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.4 +0.1 -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.9 +0.6 -0.3 +1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 +0.1 -0.1 +1.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.8 +0.1 +0.0 +1.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 +0.4 -0.0 +1.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 +0.3 +0.0 +0.7
growth on ypd YPD N.D. -0.2 +0.8 +0.4 +2.7
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