Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12339 and 12340 are separated by 598 nucleotides12340 and 12341 are separated by 870 nucleotides12341 and 12342 are separated by 31 nucleotides12342 and 12343 are separated by 725 nucleotides mRNA_3971: 12339 - K02964 RP-S18e, RPS18 small subunit ribosomal protein S18e, at 1,027,853 to 1,028,767 12339 mRNA_3972: 12340 - K11594 DDX3X, bel ATP-dependent RNA helicase DDX3X, at 1,029,366 to 1,031,642 12340 mRNA_3973: 12341 - HMMPfam-Flavin reductase like domain-PF01613,SMART-Flavin reductase like domain-SM00903,SUPERFAMILY--SSF50475, at 1,032,513 to 1,033,671 12341 mRNA_3974: 12342 - K02350 POLZ1, rev3 DNA polymerase zeta, at 1,033,703 to 1,040,837 12342 mRNA_3975: 12343 - KOG1938 Protein with predicted involvement in meiosis (GSG1), at 1,041,563 to 1,046,675 12343
Group Condition mRNA_3971 mRNA_3972 mRNA_3973 mRNA_3974 mRNA_3975
12339 12340 12341 12342 12343
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.7 +0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.3 -0.1 +0.1
growth on oleic acid YNB Glucose N.D. N.D. -0.0 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.2 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 -0.3 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.7 +0.1 +0.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.5 +0.3 +0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.3 +0.1 -0.1
growth on ypd YPD N.D. N.D. +0.1 -0.3 +0.6
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 +0.1 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.1 +0.1 +0.5
auxotrophy Dropout Complete N.D. N.D. -0.0 +0.1 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.0 +0.1 +0.6
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.1 -0.1 +0.9
auxotrophy YNB Glucose N.D. N.D. +0.2 +0.0 +0.6
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.1 +0.2 +0.6
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.1 +0.2 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.5 +0.1 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.6 -0.1 +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.7 -0.0 +0.8
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