Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12340 and 12341 are separated by 870 nucleotides12341 and 12342 are separated by 31 nucleotides12342 and 12343 are separated by 725 nucleotides12343 and 12344 are separated by 400 nucleotides mRNA_3972: 12340 - K11594 DDX3X, bel ATP-dependent RNA helicase DDX3X, at 1,029,366 to 1,031,642 12340 mRNA_3973: 12341 - HMMPfam-Flavin reductase like domain-PF01613,SMART-Flavin reductase like domain-SM00903,SUPERFAMILY--SSF50475, at 1,032,513 to 1,033,671 12341 mRNA_3974: 12342 - K02350 POLZ1, rev3 DNA polymerase zeta, at 1,033,703 to 1,040,837 12342 mRNA_3975: 12343 - KOG1938 Protein with predicted involvement in meiosis (GSG1), at 1,041,563 to 1,046,675 12343 mRNA_3976: 12344 - K20473 NBAS neuroblastoma-amplified sequence, at 1,047,076 to 1,050,963 12344
Group Condition mRNA_3972 mRNA_3973 mRNA_3974 mRNA_3975 mRNA_3976
12340 12341 12342 12343 12344
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.7 +0.0 +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 -0.1 +0.1 N.D.
growth on oleic acid YNB Glucose N.D. -0.0 -0.1 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 -0.2 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 -0.3 +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.2 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.7 +0.1 +0.7 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 +0.3 +0.5 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 +0.1 -0.1 N.D.
growth on ypd YPD N.D. +0.1 -0.3 +0.6 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.1 +0.1 +0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 +0.1 +0.5 N.D.
auxotrophy Dropout Complete N.D. -0.0 +0.1 +0.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 +0.1 +0.6 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.1 +0.9 N.D.
auxotrophy YNB Glucose N.D. +0.2 +0.0 +0.6 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 +0.2 +0.6 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.2 +0.6 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 +0.1 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 -0.1 +0.9 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.7 -0.0 +0.8 N.D.
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