Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12378 and 12379 are separated by 384 nucleotides12379 and 12380 are separated by 224 nucleotides12380 and 12381 are separated by 913 nucleotides12381 and 12382 are separated by 228 nucleotides mRNA_4010: 12378 - HMMPfam-F-box-like-PF12937,ProSiteProfiles-F-box domain profile.-PS50181,SMART-A Receptor for Ubiquitination Targets-SM00256,SMART-Leucine-rich repeat - CC (cysteine-containing) subfamily-SM00367,SUPERFAMILY--SSF52047,SUPERFAMILY--SSF81383, at 1,142,539 to 1,145,554 12378 mRNA_4011: 12379 - HMMPfam-Retinoic acid induced 16-like protein-PF10257, at 1,145,939 to 1,149,704 12379 mRNA_4012: 12380 - KOG2370 Cactin, at 1,149,929 to 1,152,487 12380 mRNA_4013: 12381 - HMMPfam-DJ-1/PfpI family-PF01965,HMMPfam-N-terminal domain of DJ-1_PfpI family-PF13587,SUPERFAMILY--SSF52317, at 1,153,401 to 1,154,671 12381 mRNA_4014: 12382 - KOG0065 Pleiotropic drug resistance proteins (PDR1-15), ABC superfamily, at 1,154,900 to 1,159,801 12382
Group Condition mRNA_4010 mRNA_4011 mRNA_4012 mRNA_4013 mRNA_4014
12378 12379 12380 12381 12382
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.1 N.D. -1.8 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.2 N.D. -0.5 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.2 N.D. -0.7 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.1 N.D. -0.2 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.5 -0.3 N.D. -0.7 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.1 N.D. -0.2 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.2 N.D. -0.1 +0.3
auxotrophy YNB Glucose -0.3 +0.1 N.D. -0.0 +0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.0 N.D. +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.5 +0.1 N.D. -0.5 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.2 N.D. +0.0 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.1 N.D. -0.3 +0.6
auxotrophy Dropout Complete -0.5 -0.0 N.D. +0.6 +0.2
growth on ricinoleic acid YNB Glucose -0.1 -0.2 N.D. +0.7 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.1 N.D. -0.1 +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.4 N.D. -0.1 +0.0
growth on oleic acid YNB Glucose +0.1 +0.1 N.D. +0.4 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.5 N.D. +1.4 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 +0.3 N.D. -0.4 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.4 N.D. +1.2 +0.2
growth on ypd YPD +0.0 +0.4 N.D. +1.8 +0.2
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