Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12381 and 12382 are separated by 228 nucleotides12382 and 12383 are separated by 162 nucleotides12383 and 12384 are separated by 39 nucleotides12384 and 12385 are separated by 456 nucleotides mRNA_4013: 12381 - HMMPfam-DJ-1/PfpI family-PF01965,HMMPfam-N-terminal domain of DJ-1_PfpI family-PF13587,SUPERFAMILY--SSF52317, at 1,153,401 to 1,154,671 12381 mRNA_4014: 12382 - KOG0065 Pleiotropic drug resistance proteins (PDR1-15), ABC superfamily, at 1,154,900 to 1,159,801 12382 mRNA_4015: 12383 - K04512 DAAM dishevelled associated activator of morphogenesis, at 1,159,964 to 1,166,346 12383 mRNA_4016: 12384 - K01250 rihA pyrimidine-specific ribonucleoside hydrolase, at 1,166,386 to 1,167,803 12384 mRNA_4017: 12385 - K13333 PLB lysophospholipase, at 1,168,260 to 1,170,390 12385
Group Condition mRNA_4013 mRNA_4014 mRNA_4015 mRNA_4016 mRNA_4017
12381 12382 12383 12384 12385
facs on lipid accumulation Sorted No BODIPY Gate -1.8 +0.2 +0.2 -0.3 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.7 +0.2 +0.0 -0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.5 +0.0 +0.1 +0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.0 +0.1 +0.3 -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.5 +0.0 -0.0 +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.1 -0.2 +0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 +0.0 +0.1 -0.1
auxotrophy YNB Glucose -0.0 +0.1 -0.1 -0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 +0.3 +0.0 -0.0 +0.3
growth on oleic acid YNB Oleic Acid +0.2 -0.2 -0.1 +0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.1 -0.0 +0.1 +0.1
growth on oleic acid YNB Glucose +0.4 +0.1 -0.2 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.3 -0.0 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.0 +0.1 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.4 -0.0 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.6 +0.0 +0.2 -0.0
growth on ricinoleic acid YNB Glucose +0.7 -0.1 +0.1 +0.1 -0.1
auxotrophy Dropout Complete +0.6 +0.2 -0.0 -0.1 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +1.4 -0.0 -0.1 +0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +1.2 +0.2 -0.2 +0.3 +0.1
growth on ypd YPD +1.8 +0.2 -0.0 -0.3 +0.4
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