Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12385 and 12386 are separated by 228 nucleotides12386 and 12387 are separated by 243 nucleotides12387 and 12388 are separated by 540 nucleotides12388 and 12389 are separated by 351 nucleotides mRNA_4017: 12385 - K13333 PLB lysophospholipase, at 1,168,260 to 1,170,390 12385 mRNA_4018: 12386 - K09500 CCT8 T-complex protein 1 subunit theta, at 1,170,619 to 1,173,012 12386 mRNA_4019: 12387 - K15116 SLC25A33_36, RIM2 solute carrier family 25, member 33/36, at 1,173,256 to 1,174,830 12387 mRNA_4020: 12388 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 1,175,371 to 1,177,863 12388 mRNA_4021: 12389 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 1,178,215 to 1,180,562 12389
Group Condition mRNA_4017 mRNA_4018 mRNA_4019 mRNA_4020 mRNA_4021
12385 12386 12387 12388 12389
growth on ricinoleic acid YNB Glucose -0.1 N.D. N.D. -0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. N.D. -0.2 -0.3
growth on oleic acid YNB Glucose -0.3 N.D. N.D. +0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. -0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. N.D. -0.1 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. N.D. -0.1 +0.1
auxotrophy Dropout Complete +0.0 N.D. N.D. +0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. N.D. +0.2 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 N.D. N.D. +0.3 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. N.D. +0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. N.D. -0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. N.D. +0.0 +0.0
auxotrophy YNB Glucose -0.1 N.D. N.D. -0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. N.D. -0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. +0.1 -0.1
growth on oleic acid YNB Oleic Acid -0.0 N.D. N.D. +0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. +0.3 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. N.D. +0.4 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. N.D. +0.1 -0.1
growth on ypd YPD +0.4 N.D. N.D. +0.1 -0.0
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