Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12396 and 12397 are separated by 182 nucleotides12397 and 12398 are separated by 451 nucleotides12398 and 12399 are separated by 466 nucleotides12399 and 12400 are separated by 2682 nucleotides mRNA_4028: 12396 - K02328 POLD2 DNA polymerase delta subunit 2, at 1,190,339 to 1,192,414 12396 mRNA_4029: 12397 - HMMPfam-DASH complex subunit Hsk3 like-PF08227, at 1,192,597 to 1,192,970 12397 mRNA_4030: 12398 - Hypothetical Protein, at 1,193,422 to 1,193,864 12398 mRNA_4031: 12399 - K08726 EPHX2 soluble epoxide hydrolase / lipid-phosphate phosphatase, at 1,194,331 to 1,195,759 12399 mRNA_4032: 12400 - Hypothetical Protein, at 1,198,442 to 1,200,513 12400
Group Condition mRNA_4028 mRNA_4029 mRNA_4030 mRNA_4031 mRNA_4032
12396 12397 12398 12399 12400
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.7 -1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.2 +0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. +0.2 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.0 +0.1
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.3 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. +0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. +0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.3 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.2 +0.1
auxotrophy Dropout Complete N.D. N.D. N.D. +0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.2 +0.2
auxotrophy YNB Glucose N.D. N.D. N.D. +0.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.2 +0.3
growth on ypd YPD N.D. N.D. N.D. +0.0 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.2 +0.4
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