Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12400 and 12401 are separated by 269 nucleotides12401 and 12402 are separated by 1750 nucleotides12402 and 12403 are separated by 803 nucleotides12403 and 12404 are separated by 140 nucleotides mRNA_4032: 12400 - Hypothetical Protein, at 1,198,442 to 1,200,513 12400 mRNA_4033: 12401 - K17675 SUPV3L1, SUV3 ATP-dependent RNA helicase SUPV3L1/SUV3, at 1,200,783 to 1,202,735 12401 mRNA_4034: 12402 - K13181 DDX27, DRS1 ATP-dependent RNA helicase DDX27, at 1,204,486 to 1,207,026 12402 mRNA_4035: 12403 - Hypothetical Protein, at 1,207,830 to 1,209,196 12403 mRNA_4036: 12404 - Hypothetical Protein, at 1,209,337 to 1,209,939 12404
Group Condition mRNA_4032 mRNA_4033 mRNA_4034 mRNA_4035 mRNA_4036
12400 12401 12402 12403 12404
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. N.D. N.D. -1.0
growth on oleic acid YNB Glucose -0.2 N.D. N.D. N.D. -0.7
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. N.D. N.D. -0.7
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 N.D. N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. N.D. -0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. N.D. -0.5
growth on oleic acid YNB Oleic Acid -0.0 N.D. N.D. N.D. -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. N.D. N.D. -0.7
auxotrophy YNB Glucose +0.2 N.D. N.D. N.D. -0.8
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. N.D. N.D. -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. N.D. N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. N.D. -0.5
auxotrophy Dropout Complete +0.3 N.D. N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. N.D. N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. N.D. N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. N.D. -0.2
growth on ypd YPD +0.6 N.D. N.D. N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. N.D. N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. N.D. N.D. +0.3
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