Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12401 and 12402 are separated by 1750 nucleotides12402 and 12403 are separated by 803 nucleotides12403 and 12404 are separated by 140 nucleotides12404 and 12405 are separated by 212 nucleotides mRNA_4033: 12401 - K17675 SUPV3L1, SUV3 ATP-dependent RNA helicase SUPV3L1/SUV3, at 1,200,783 to 1,202,735 12401 mRNA_4034: 12402 - K13181 DDX27, DRS1 ATP-dependent RNA helicase DDX27, at 1,204,486 to 1,207,026 12402 mRNA_4035: 12403 - Hypothetical Protein, at 1,207,830 to 1,209,196 12403 mRNA_4036: 12404 - Hypothetical Protein, at 1,209,337 to 1,209,939 12404 mRNA_4037: 12405 - K12232 HECTD2 E3 ubiquitin-protein ligase HECTD2, at 1,210,152 to 1,214,937 12405
Group Condition mRNA_4033 mRNA_4034 mRNA_4035 mRNA_4036 mRNA_4037
12401 12402 12403 12404 12405
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -1.0 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.7 -0.1
auxotrophy YNB Glucose N.D. N.D. N.D. -0.8 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.9 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.5 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.7 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.4 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.5 +0.1
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.7 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.3 -0.1
growth on ypd YPD N.D. N.D. N.D. -0.3 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.5 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.3 -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.6 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.4 +0.3
auxotrophy Dropout Complete N.D. N.D. N.D. -0.4 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.3 +0.2
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