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Protein
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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12402 and 12403 are separated by 803 nucleotides
12403 and 12404 are separated by 140 nucleotides
12404 and 12405 are separated by 212 nucleotides
12405 and 12406 are separated by 92 nucleotides
mRNA_4034: 12402 - K13181 DDX27, DRS1 ATP-dependent RNA helicase DDX27, at 1,204,486 to 1,207,026
12402
mRNA_4035: 12403 - Hypothetical Protein, at 1,207,830 to 1,209,196
12403
mRNA_4036: 12404 - Hypothetical Protein, at 1,209,337 to 1,209,939
12404
mRNA_4037: 12405 - K12232 HECTD2 E3 ubiquitin-protein ligase HECTD2, at 1,210,152 to 1,214,937
12405
mRNA_4038: 12406 - K10772 APEX2 AP endonuclease 2, at 1,215,030 to 1,217,455
12406
Group
Condition
mRNA
_4034
mRNA
_4035
mRNA
_4036
mRNA
_4037
mRNA
_4038
12402
12403
12404
12405
12406
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
N.D.
-0.7
-0.1
+0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
N.D.
-1.0
+0.1
+0.1
auxotrophy
YNB Glucose
N.D.
N.D.
-0.8
+0.1
-0.0
growth on ricinoleic acid
YNB Glucose
N.D.
N.D.
-0.9
+0.3
-0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
N.D.
-0.3
-0.1
-0.2
growth on oleic acid
YNB Glucose
N.D.
N.D.
-0.7
+0.3
-0.2
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
N.D.
-0.4
-0.2
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
N.D.
-0.3
-0.1
-0.2
growth on oleic acid
YNB Oleic Acid
N.D.
N.D.
-0.6
+0.3
-0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
N.D.
-0.5
-0.1
+0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
N.D.
-0.4
+0.3
-0.2
auxotrophy
YNB Glucose 75mM Methionine
N.D.
N.D.
-0.5
+0.1
+0.1
auxotrophy
YNB Glucose 75mM Arginine
N.D.
N.D.
-0.5
+0.2
+0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
N.D.
-0.7
+0.1
+0.4
growth on ypd
YPD
N.D.
N.D.
-0.3
-0.0
+0.2
ypd buoyancy
40 hrs Low Buoyancy
N.D.
N.D.
-0.1
+0.1
-0.1
ypd buoyancy
40 hrs Median Buoyancy
N.D.
N.D.
-0.2
+0.1
+0.0
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
N.D.
+0.1
-0.2
+0.2
auxotrophy
Dropout Complete
N.D.
N.D.
-0.4
+0.3
+0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
N.D.
-0.0
-0.1
+0.5
ypd buoyancy
40 hrs High Buoyancy
N.D.
N.D.
+0.3
+0.2
+0.2
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