Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12402 and 12403 are separated by 803 nucleotides12403 and 12404 are separated by 140 nucleotides12404 and 12405 are separated by 212 nucleotides12405 and 12406 are separated by 92 nucleotides mRNA_4034: 12402 - K13181 DDX27, DRS1 ATP-dependent RNA helicase DDX27, at 1,204,486 to 1,207,026 12402 mRNA_4035: 12403 - Hypothetical Protein, at 1,207,830 to 1,209,196 12403 mRNA_4036: 12404 - Hypothetical Protein, at 1,209,337 to 1,209,939 12404 mRNA_4037: 12405 - K12232 HECTD2 E3 ubiquitin-protein ligase HECTD2, at 1,210,152 to 1,214,937 12405 mRNA_4038: 12406 - K10772 APEX2 AP endonuclease 2, at 1,215,030 to 1,217,455 12406
Group Condition mRNA_4034 mRNA_4035 mRNA_4036 mRNA_4037 mRNA_4038
12402 12403 12404 12405 12406
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.7 -0.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -1.0 +0.1 +0.1
auxotrophy YNB Glucose N.D. N.D. -0.8 +0.1 -0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.9 +0.3 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.3 -0.1 -0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.7 +0.3 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.4 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.3 -0.1 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.6 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.5 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.4 +0.3 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.5 +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.5 +0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.7 +0.1 +0.4
growth on ypd YPD N.D. N.D. -0.3 -0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.2 +0.1 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.1 -0.2 +0.2
auxotrophy Dropout Complete N.D. N.D. -0.4 +0.3 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.0 -0.1 +0.5
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.3 +0.2 +0.2
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