Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12405 and 12406 are separated by 92 nucleotides12406 and 12407 are separated by 305 nucleotides12407 and 12408 are separated by 141 nucleotides12408 and 12409 are separated by 394 nucleotides mRNA_4037: 12405 - K12232 HECTD2 E3 ubiquitin-protein ligase HECTD2, at 1,210,152 to 1,214,937 12405 mRNA_4038: 12406 - K10772 APEX2 AP endonuclease 2, at 1,215,030 to 1,217,455 12406 mRNA_4039: 12407 - K00825 AADAT, KAT2 kynurenine/2-aminoadipate aminotransferase, at 1,217,761 to 1,219,740 12407 mRNA_4040: 12408 - K06997 yggS, PROSC PLP dependent protein, at 1,219,882 to 1,220,973 12408 mRNA_4041: 12409 - HMMPfam-Sds3-like-PF08598, at 1,221,368 to 1,222,599 12409
Group Condition mRNA_4037 mRNA_4038 mRNA_4039 mRNA_4040 mRNA_4041
12405 12406 12407 12408 12409
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.5 -0.3 -0.4 -4.6
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.0 -0.2 -0.2 -3.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.2 -0.3 -0.3 -2.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.2 -0.0 -0.3 -2.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 -0.3 -0.2 -2.0
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.1 +0.1 -0.0 -1.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.2 +0.1 -0.4 -0.4
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 +0.4 -0.1 -1.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.1 +0.2 -0.8 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.4 -0.2 -0.9 +0.6
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 -0.1 +0.1 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.1 +0.5 -0.0 -0.4
growth on ypd YPD -0.0 +0.2 +1.1 +0.2 -1.1
auxotrophy YNB Glucose +0.1 -0.0 +0.1 +0.1 +0.2
growth on ricinoleic acid YNB Glucose +0.3 -0.0 -0.2 +0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.2 -0.7 +0.1 +1.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.0 -0.0 -0.3 +1.0
auxotrophy Dropout Complete +0.3 +0.3 +0.3 +0.0 +0.0
growth on oleic acid YNB Oleic Acid +0.3 -0.2 +0.3 +0.1 +0.7
growth on oleic acid YNB Glucose +0.3 -0.2 +0.3 +0.0 +1.1
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.2 -0.1 -0.0 +1.2
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