Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12406 and 12407 are separated by 305 nucleotides12407 and 12408 are separated by 141 nucleotides12408 and 12409 are separated by 394 nucleotides12409 and 12410 are separated by 125 nucleotides mRNA_4038: 12406 - K10772 APEX2 AP endonuclease 2, at 1,215,030 to 1,217,455 12406 mRNA_4039: 12407 - K00825 AADAT, KAT2 kynurenine/2-aminoadipate aminotransferase, at 1,217,761 to 1,219,740 12407 mRNA_4040: 12408 - K06997 yggS, PROSC PLP dependent protein, at 1,219,882 to 1,220,973 12408 mRNA_4041: 12409 - HMMPfam-Sds3-like-PF08598, at 1,221,368 to 1,222,599 12409 mRNA_4042: 12410 - KOG0807 Carbon-nitrogen hydrolase, at 1,222,725 to 1,223,895 12410
Group Condition mRNA_4038 mRNA_4039 mRNA_4040 mRNA_4041 mRNA_4042
12406 12407 12408 12409 12410
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 -0.3 -0.4 -4.6 -0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.2 -0.2 -3.3 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.3 -0.3 -2.8 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.0 -0.3 -2.5 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.3 -0.2 -2.0 +0.6
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 -0.0 -1.4 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.4 -0.1 -1.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.1 -0.4 -0.4 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.2 -0.8 +0.3 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.5 -0.0 -0.4 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.2 -0.9 +0.6 +0.0
auxotrophy YNB Glucose -0.0 +0.1 +0.1 +0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 +0.1 +0.0 +0.3
growth on ypd YPD +0.2 +1.1 +0.2 -1.1 -0.0
auxotrophy Dropout Complete +0.3 +0.3 +0.0 +0.0 -0.2
growth on ricinoleic acid YNB Glucose -0.0 -0.2 +0.2 +0.2 +0.5
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.0 -0.3 +1.0 +0.2
growth on oleic acid YNB Oleic Acid -0.2 +0.3 +0.1 +0.7 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.1 -0.0 +1.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.7 +0.1 +1.3 +0.7
growth on oleic acid YNB Glucose -0.2 +0.3 +0.0 +1.1 +0.6
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