Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12407 and 12408 are separated by 141 nucleotides12408 and 12409 are separated by 394 nucleotides12409 and 12410 are separated by 125 nucleotides12410 and 12411 are separated by 539 nucleotides mRNA_4039: 12407 - K00825 AADAT, KAT2 kynurenine/2-aminoadipate aminotransferase, at 1,217,761 to 1,219,740 12407 mRNA_4040: 12408 - K06997 yggS, PROSC PLP dependent protein, at 1,219,882 to 1,220,973 12408 mRNA_4041: 12409 - HMMPfam-Sds3-like-PF08598, at 1,221,368 to 1,222,599 12409 mRNA_4042: 12410 - KOG0807 Carbon-nitrogen hydrolase, at 1,222,725 to 1,223,895 12410 mRNA_4043: 12411 - Hypothetical Protein, at 1,224,435 to 1,225,613 12411
Group Condition mRNA_4039 mRNA_4040 mRNA_4041 mRNA_4042 mRNA_4043
12407 12408 12409 12410 12411
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.4 -4.6 -0.5 -1.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.3 -2.8 +0.3 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.2 -3.3 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.3 -2.5 +0.2 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.2 -2.0 +0.6 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.1 -1.0 -0.3 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.9 +0.6 +0.0 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.4 -0.4 +0.2 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.0 -1.4 +0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.8 +0.3 -0.2 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.0 -0.4 -0.4 +0.0
auxotrophy YNB Glucose +0.1 +0.1 +0.2 -0.1 -0.3
growth on ypd YPD +1.1 +0.2 -1.1 -0.0 +0.2
growth on ricinoleic acid YNB Glucose -0.2 +0.2 +0.2 +0.5 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.1 +0.0 +0.3 +0.3
auxotrophy Dropout Complete +0.3 +0.0 +0.0 -0.2 +0.5
growth on oleic acid YNB Oleic Acid +0.3 +0.1 +0.7 +0.3 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.3 +1.0 +0.2 +0.6
growth on oleic acid YNB Glucose +0.3 +0.0 +1.1 +0.6 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 +0.1 +1.3 +0.7 +0.6
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 +1.2 +0.1 +0.8
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