Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12418 and 12419 are separated by 1460 nucleotides12419 and 12420 are separated by 400 nucleotides12420 and 12421 are separated by 381 nucleotides12421 and 12422 are separated by 163 nucleotides mRNA_4050: 12418 - K02885 RP-L19e, RPL19 large subunit ribosomal protein L19e, at 1,239,655 to 1,240,764 12418 mRNA_4051: 12419 - HMMPfam-Rare lipoprotein A (RlpA)-like double-psi beta-barrel-PF03330,SUPERFAMILY--SSF50685, at 1,242,225 to 1,242,676 12419 mRNA_4052: 12420 - HMMPfam-Transcription factor Opi1-PF08618, at 1,243,077 to 1,245,269 12420 mRNA_4053: 12421 - K00602 purH phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase, at 1,245,651 to 1,248,069 12421 mRNA_4054: 12422 - KOG2973 Uncharacterized conserved protein, at 1,248,233 to 1,250,054 12422
Group Condition mRNA_4050 mRNA_4051 mRNA_4052 mRNA_4053 mRNA_4054
12418 12419 12420 12421 12422
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.0 -1.0 N.D. -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -1.2 -0.5 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 -0.0 N.D. -0.7
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 -0.5 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 +0.1 N.D. -1.5
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.2 N.D. -0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.1 N.D. -0.6
growth on ypd YPD N.D. -0.6 -0.0 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.4 +0.3 N.D. -1.0
growth on oleic acid YNB Oleic Acid N.D. -0.3 +0.0 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.5 -0.2 N.D. -0.6
growth on oleic acid YNB Glucose N.D. +0.1 +0.2 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.6 -0.9 N.D. +0.3
growth on ricinoleic acid YNB Glucose N.D. +0.5 +0.1 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 +0.2 N.D. -0.3
auxotrophy Dropout Complete N.D. +0.4 +0.3 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 +0.7 N.D. -0.1
auxotrophy YNB Glucose N.D. +0.3 +0.3 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 +0.9 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +1.0 +0.9 N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 +2.6 N.D. -0.4
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