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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12428 and 12429 are separated by 170 nucleotides
12429 and 12430 are separated by 580 nucleotides
12430 and 12431 are separated by 147 nucleotides
12431 and 12432 are separated by 105 nucleotides
mRNA_4060: 12428 - K01537 E3.6.3.8 Ca2+-transporting ATPase, at 1,257,194 to 1,260,711
12428
mRNA_4061: 12429 - SUPERFAMILY--SSF52540, at 1,260,882 to 1,262,989
12429
mRNA_4062: 12430 - HMMPfam-Protein of unknown function (DUF1115)-PF06544, at 1,263,570 to 1,264,744
12430
mRNA_4063: 12431 - K10777 LIG4, DNL4 DNA ligase 4, at 1,264,892 to 1,269,621
12431
mRNA_4064: 12432 - HMMPfam-Rdx family-PF10262,SUPERFAMILY--SSF52833, at 1,269,727 to 1,270,597
12432
Group
Condition
mRNA
_4060
mRNA
_4061
mRNA
_4062
mRNA
_4063
mRNA
_4064
12428
12429
12430
12431
12432
facs on lipid accumulation
Sorted Low BODIPY Gate
-1.9
-0.0
-0.1
+0.3
+0.5
ypd buoyancy
40 hrs No Buoyancy Separation
-0.4
-0.3
+0.0
-0.4
+0.2
ypd buoyancy
40 hrs High Buoyancy
-0.8
-0.0
-0.2
-0.3
+0.7
facs on lipid accumulation
Sorted No BODIPY Gate
-0.7
+0.3
+0.0
+0.0
+0.1
growth on oleic acid
YNB Glucose
-0.3
-0.1
+0.0
+0.2
+0.2
auxotrophy
YNB Glucose 75mM Methionine
-0.6
+0.0
+0.4
-0.1
+0.2
auxotrophy
YNB Glucose 75mM Arginine
-0.1
-0.1
+0.4
-0.1
+0.1
auxotrophy
Dropout Complete
+0.0
-0.1
+0.2
-0.1
+0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.0
-0.2
+0.2
+0.1
+0.1
auxotrophy
YNB Glucose
+0.3
-0.1
+0.3
-0.1
+0.2
growth on ypd
YPD
-0.1
+0.1
+0.3
+0.6
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.7
+0.1
+0.1
+0.1
+0.2
ypd buoyancy
40 hrs Low Buoyancy
+0.2
+0.1
+0.1
-0.1
+0.8
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.6
+0.0
-0.1
+0.2
+0.4
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+1.0
+0.2
-0.3
+0.2
+0.3
growth on ricinoleic acid
YNB Glucose
+0.8
+0.4
+0.1
+0.3
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
+0.5
+0.2
-0.1
+0.2
+0.8
growth on oleic acid
YNB Oleic Acid
+1.0
+0.1
+0.4
+0.2
+0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
+1.0
+0.2
+0.0
+0.3
+0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
+1.2
+0.3
+0.6
+0.2
-0.5
growth on ricinoleic acid
YNB Methylricinoleic Acid
+1.5
+0.4
+0.4
+0.4
-0.2
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