Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12430 and 12431 are separated by 147 nucleotides12431 and 12432 are separated by 105 nucleotides12432 and 12433 are separated by 138 nucleotides12433 and 12434 are separated by 946 nucleotides mRNA_4062: 12430 - HMMPfam-Protein of unknown function (DUF1115)-PF06544, at 1,263,570 to 1,264,744 12430 mRNA_4063: 12431 - K10777 LIG4, DNL4 DNA ligase 4, at 1,264,892 to 1,269,621 12431 mRNA_4064: 12432 - HMMPfam-Rdx family-PF10262,SUPERFAMILY--SSF52833, at 1,269,727 to 1,270,597 12432 mRNA_4065: 12433 - KOG1198 Zinc-binding oxidoreductase, at 1,270,736 to 1,272,325 12433 mRNA_4066: 12434 - K03426 E3.6.1.22, NUDT12, nudC NAD+ diphosphatase, at 1,273,272 to 1,275,258 12434
Group Condition mRNA_4062 mRNA_4063 mRNA_4064 mRNA_4065 mRNA_4066
12430 12431 12432 12433 12434
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.4 +0.2 -1.7 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.0 +0.1 -0.8 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.2 +0.8 -1.7 +0.1
growth on ypd YPD +0.3 +0.6 +0.1 -1.5 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.3 +0.7 -0.5 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.2 +0.3 +0.7 -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.4 -0.2 +0.3 -0.5
growth on ricinoleic acid YNB Glucose +0.1 +0.3 -0.1 +0.6 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 +0.2 +0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.1 +0.1 +1.1 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 +0.2 -0.5 +0.9 -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 +0.8 -0.2 +0.3
growth on oleic acid YNB Glucose +0.0 +0.2 +0.2 +0.4 +0.1
growth on oleic acid YNB Oleic Acid +0.4 +0.2 +0.1 +0.1 +0.1
auxotrophy YNB Glucose +0.3 -0.1 +0.2 +1.1 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.3 +0.5 +0.1 +0.7
auxotrophy Dropout Complete +0.2 -0.1 +0.2 +1.7 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.2 +0.4 +1.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.3 +0.2 +0.8 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.1 +0.1 +1.2 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.1 +0.2 +1.7 -0.5
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