Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12431 and 12432 are separated by 105 nucleotides12432 and 12433 are separated by 138 nucleotides12433 and 12434 are separated by 946 nucleotides12434 and 12435 are separated by 131 nucleotides mRNA_4063: 12431 - K10777 LIG4, DNL4 DNA ligase 4, at 1,264,892 to 1,269,621 12431 mRNA_4064: 12432 - HMMPfam-Rdx family-PF10262,SUPERFAMILY--SSF52833, at 1,269,727 to 1,270,597 12432 mRNA_4065: 12433 - KOG1198 Zinc-binding oxidoreductase, at 1,270,736 to 1,272,325 12433 mRNA_4066: 12434 - K03426 E3.6.1.22, NUDT12, nudC NAD+ diphosphatase, at 1,273,272 to 1,275,258 12434 mRNA_4067: 12435 - KOG0324 Uncharacterized conserved protein, at 1,275,390 to 1,276,654 12435
Group Condition mRNA_4063 mRNA_4064 mRNA_4065 mRNA_4066 mRNA_4067
12431 12432 12433 12434 12435
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.2 -1.7 +0.1 +0.3
growth on ypd YPD +0.6 +0.1 -1.5 -0.1 -0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.8 -1.7 +0.1 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.1 -0.8 -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 +1.1 -0.6 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.7 -0.5 +0.1 +0.2
growth on oleic acid YNB Glucose +0.2 +0.2 +0.4 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.3 +0.7 -0.6 +0.3
growth on oleic acid YNB Oleic Acid +0.2 +0.1 +0.1 +0.1 +0.4
auxotrophy Dropout Complete -0.1 +0.2 +1.7 -0.5 -0.4
auxotrophy YNB Glucose -0.1 +0.2 +1.1 -0.4 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.2 +0.3 -0.5 +0.9
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.5 +0.9 -0.4 +0.7
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.8 -0.2 +0.3 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 +0.1 +0.3 +0.4
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 +1.7 -0.5 -0.2
growth on ricinoleic acid YNB Glucose +0.3 -0.1 +0.6 -0.3 +0.9
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.5 +0.1 +0.7 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.1 +1.2 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.2 +0.8 +0.3 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.4 +1.1 -0.3 +0.8
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