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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12431 and 12432 are separated by 105 nucleotides
12432 and 12433 are separated by 138 nucleotides
12433 and 12434 are separated by 946 nucleotides
12434 and 12435 are separated by 131 nucleotides
mRNA_4063: 12431 - K10777 LIG4, DNL4 DNA ligase 4, at 1,264,892 to 1,269,621
12431
mRNA_4064: 12432 - HMMPfam-Rdx family-PF10262,SUPERFAMILY--SSF52833, at 1,269,727 to 1,270,597
12432
mRNA_4065: 12433 - KOG1198 Zinc-binding oxidoreductase, at 1,270,736 to 1,272,325
12433
mRNA_4066: 12434 - K03426 E3.6.1.22, NUDT12, nudC NAD+ diphosphatase, at 1,273,272 to 1,275,258
12434
mRNA_4067: 12435 - KOG0324 Uncharacterized conserved protein, at 1,275,390 to 1,276,654
12435
Group
Condition
mRNA
_4063
mRNA
_4064
mRNA
_4065
mRNA
_4066
mRNA
_4067
12431
12432
12433
12434
12435
ypd buoyancy
40 hrs No Buoyancy Separation
-0.4
+0.2
-1.7
+0.1
+0.3
growth on ypd
YPD
+0.6
+0.1
-1.5
-0.1
-0.5
facs on lipid accumulation
Sorted High BODIPY Gate
+0.2
+0.8
-1.7
+0.1
-0.3
facs on lipid accumulation
Sorted No BODIPY Gate
+0.0
+0.1
-0.8
-0.2
-0.1
auxotrophy
YNB Glucose 75mM Arginine
-0.1
+0.1
+1.1
-0.6
-0.3
ypd buoyancy
40 hrs High Buoyancy
-0.3
+0.7
-0.5
+0.1
+0.2
growth on oleic acid
YNB Glucose
+0.2
+0.2
+0.4
+0.1
-0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.2
+0.3
+0.7
-0.6
+0.3
growth on oleic acid
YNB Oleic Acid
+0.2
+0.1
+0.1
+0.1
+0.4
auxotrophy
Dropout Complete
-0.1
+0.2
+1.7
-0.5
-0.4
auxotrophy
YNB Glucose
-0.1
+0.2
+1.1
-0.4
+0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.4
-0.2
+0.3
-0.5
+0.9
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.2
-0.5
+0.9
-0.4
+0.7
ypd buoyancy
40 hrs Low Buoyancy
-0.1
+0.8
-0.2
+0.3
+0.3
ypd buoyancy
40 hrs Median Buoyancy
+0.1
+0.2
+0.1
+0.3
+0.4
auxotrophy
YNB Glucose 75mM Methionine
-0.1
+0.2
+1.7
-0.5
-0.2
growth on ricinoleic acid
YNB Glucose
+0.3
-0.1
+0.6
-0.3
+0.9
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.3
+0.5
+0.1
+0.7
+0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
+0.1
+1.2
+0.1
+0.4
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.3
+0.2
+0.8
+0.3
+0.4
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.2
+0.4
+1.1
-0.3
+0.8
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