Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12433 and 12434 are separated by 946 nucleotides12434 and 12435 are separated by 131 nucleotides12435 and 12436 are separated by 539 nucleotides12436 and 12437 are separated by 171 nucleotides mRNA_4065: 12433 - KOG1198 Zinc-binding oxidoreductase, at 1,270,736 to 1,272,325 12433 mRNA_4066: 12434 - K03426 E3.6.1.22, NUDT12, nudC NAD+ diphosphatase, at 1,273,272 to 1,275,258 12434 mRNA_4067: 12435 - KOG0324 Uncharacterized conserved protein, at 1,275,390 to 1,276,654 12435 mRNA_4068: 12436 - K02575 NRT, narK, nrtP, nasA MFS transporter, NNP family, nitrate/nitrite transporter, at 1,277,194 to 1,279,271 12436 mRNA_4069: 12437 - K05288 PIGO phosphatidylinositol glycan, class O, at 1,279,443 to 1,282,832 12437
Group Condition mRNA_4065 mRNA_4066 mRNA_4067 mRNA_4068 mRNA_4069
12433 12434 12435 12436 12437
growth on ypd YPD -1.5 -0.1 -0.5 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.7 +0.1 +0.3 -0.6 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -1.7 +0.1 -0.3 +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.8 -0.2 -0.1 +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.5 +0.1 +0.2 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 -0.6 +0.3 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.3 +0.3 +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +1.1 -0.6 -0.3 +0.3 N.D.
auxotrophy YNB Glucose +1.1 -0.4 +0.0 -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.7 +0.1 -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.5 +0.9 +0.0 N.D.
growth on oleic acid YNB Glucose +0.4 +0.1 -0.2 +0.5 N.D.
growth on oleic acid YNB Oleic Acid +0.1 +0.1 +0.4 +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.3 +0.4 +0.3 N.D.
auxotrophy Dropout Complete +1.7 -0.5 -0.4 +0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine +1.7 -0.5 -0.2 +0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.6 -0.3 +0.9 +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 -0.4 +0.7 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.1 -0.3 +0.8 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 +0.3 +0.4 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.2 +0.1 +0.4 +0.5 N.D.
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