Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12437 and 12438 are separated by 678 nucleotides12438 and 12439 are separated by 707 nucleotides12439 and 12440 are separated by 683 nucleotides12440 and 12441 are separated by 1147 nucleotides mRNA_4069: 12437 - K05288 PIGO phosphatidylinositol glycan, class O, at 1,279,443 to 1,282,832 12437 mRNA_4070: 12438 - K02877 RP-L15e, RPL15 large subunit ribosomal protein L15e, at 1,283,511 to 1,284,371 12438 mRNA_4071: 12439 - BLAST putative upf0132 domain-containing protein [Diaporthe..., at 1,285,079 to 1,285,837 12439 mRNA_4072: 12440 - K16575 ACTR1, ARP1 centractin, at 1,286,521 to 1,288,236 12440 mRNA_4073: 12441 - KOG0961 Predicted Zn2+-dependent endopeptidase, insulinase superfamily, at 1,289,384 to 1,292,413 12441
Group Condition mRNA_4069 mRNA_4070 mRNA_4071 mRNA_4072 mRNA_4073
12437 12438 12439 12440 12441
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.0 N.D. -0.7
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 N.D. -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.0 N.D. -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.0 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.3 N.D. -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.3 N.D. -0.5
auxotrophy Dropout Complete N.D. N.D. -0.0 N.D. -0.1
growth on oleic acid YNB Glucose N.D. N.D. +0.3 N.D. -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.1 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.3 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 N.D. +0.2
auxotrophy YNB Glucose N.D. N.D. +0.3 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.3 N.D. +0.2
growth on ypd YPD N.D. N.D. +0.7 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.2 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.5 N.D. +0.1
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