Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12470 and 12471 are separated by 240 nucleotides12471 and 12472 are separated by 61 nucleotides12472 and 12473 are separated by 205 nucleotides12473 and 12474 are separated by 552 nucleotides mRNA_4102: 12470 - K01889 FARSA, pheS phenylalanyl-tRNA synthetase alpha chain, at 1,362,795 to 1,364,929 12470 mRNA_4103: 12471 - K00326 E1.6.2.2 cytochrome-b5 reductase, at 1,365,170 to 1,366,480 12471 mRNA_4104: 12472 - K06664 PEX2, PXMP3 peroxin-2, at 1,366,542 to 1,368,215 12472 mRNA_4105: 12473 - K15306 RANBP1 Ran-binding protein 1, at 1,368,421 to 1,369,571 12473 mRNA_4106: 12474 - HMMPfam-L-lysine 6-monooxygenase (NADPH-requiring)-PF13434,SUPERFAMILY--SSF51905, at 1,370,124 to 1,372,356 12474
Group Condition mRNA_4102 mRNA_4103 mRNA_4104 mRNA_4105 mRNA_4106
12470 12471 12472 12473 12474
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. N.D. -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. N.D. -0.4
growth on ypd YPD N.D. N.D. N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. N.D. -0.0
auxotrophy YNB Glucose N.D. N.D. N.D. N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. N.D. +0.0
auxotrophy Dropout Complete N.D. N.D. N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. N.D. +0.1
growth on oleic acid YNB Glucose N.D. N.D. N.D. N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. N.D. +0.2
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