Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12472 and 12473 are separated by 205 nucleotides12473 and 12474 are separated by 552 nucleotides12474 and 12475 are separated by 211 nucleotides12475 and 12476 are separated by 141 nucleotides mRNA_4104: 12472 - K06664 PEX2, PXMP3 peroxin-2, at 1,366,542 to 1,368,215 12472 mRNA_4105: 12473 - K15306 RANBP1 Ran-binding protein 1, at 1,368,421 to 1,369,571 12473 mRNA_4106: 12474 - HMMPfam-L-lysine 6-monooxygenase (NADPH-requiring)-PF13434,SUPERFAMILY--SSF51905, at 1,370,124 to 1,372,356 12474 mRNA_4107: 12475 - K00621 GNPNAT1, GNA1 glucosamine-phosphate N-acetyltransferase, at 1,372,568 to 1,373,212 12475 mRNA_4108: 12476 - HMMPfam-Aminotransferase class IV-PF01063,SUPERFAMILY--SSF56752, at 1,373,354 to 1,374,334 12476
Group Condition mRNA_4104 mRNA_4105 mRNA_4106 mRNA_4107 mRNA_4108
12472 12473 12474 12475 12476
growth on ypd YPD N.D. N.D. -0.4 N.D. -0.8
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.3 N.D. -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.4 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 N.D. -0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.2 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.4 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.0 N.D. -0.1
auxotrophy Dropout Complete N.D. N.D. +0.1 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.0 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.1 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.2 N.D. -0.0
auxotrophy YNB Glucose N.D. N.D. +0.0 N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.3 N.D. +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.5 N.D. +0.8
growth on oleic acid YNB Glucose N.D. N.D. +0.1 N.D. +0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 N.D. +0.8
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