Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12473 and 12474 are separated by 552 nucleotides12474 and 12475 are separated by 211 nucleotides12475 and 12476 are separated by 141 nucleotides12476 and 12477 are separated by 418 nucleotides mRNA_4105: 12473 - K15306 RANBP1 Ran-binding protein 1, at 1,368,421 to 1,369,571 12473 mRNA_4106: 12474 - HMMPfam-L-lysine 6-monooxygenase (NADPH-requiring)-PF13434,SUPERFAMILY--SSF51905, at 1,370,124 to 1,372,356 12474 mRNA_4107: 12475 - K00621 GNPNAT1, GNA1 glucosamine-phosphate N-acetyltransferase, at 1,372,568 to 1,373,212 12475 mRNA_4108: 12476 - HMMPfam-Aminotransferase class IV-PF01063,SUPERFAMILY--SSF56752, at 1,373,354 to 1,374,334 12476 mRNA_4109: 12477 - Hypothetical Protein, at 1,374,753 to 1,375,099 12477
Group Condition mRNA_4105 mRNA_4106 mRNA_4107 mRNA_4108 mRNA_4109
12473 12474 12475 12476 12477
growth on ypd YPD N.D. -0.4 N.D. -0.8 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 N.D. -0.0 -1.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 N.D. -0.1 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 N.D. -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 N.D. +0.8 -1.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. -0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 N.D. -0.3 +0.0
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. +0.2 -0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 N.D. +0.6 -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 N.D. -0.1 -0.3
auxotrophy YNB Glucose N.D. +0.0 N.D. +0.1 -0.5
growth on oleic acid YNB Glucose N.D. +0.1 N.D. +0.2 -0.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 N.D. -0.4 -0.0
auxotrophy Dropout Complete N.D. +0.1 N.D. -0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 N.D. -0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. -0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.4 N.D. +0.2 +0.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 N.D. -0.4 +1.3
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 N.D. -0.5 +1.2
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. +0.8 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. -0.1 +1.2
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