Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12493 and 12494 are separated by 911 nucleotides12494 and 12495 are separated by 660 nucleotides12495 and 12496 are separated by 64 nucleotides12496 and 12497 are separated by 796 nucleotides mRNA_4125: 12493 - KOG0627 Heat shock transcription factor, at 1,407,768 to 1,409,382 12493 mRNA_4126: 12494 - K20408 WDR24 WD repeat-containing protein 24, at 1,410,294 to 1,414,666 12494 mRNA_4127: 12495 - K00793 ribE, RIB5 riboflavin synthase, at 1,415,327 to 1,416,353 12495 mRNA_4128: 12496 - K02926 RP-L4, MRPL4, rplD large subunit ribosomal protein L4, at 1,416,418 to 1,417,570 12496 mRNA_4129: 12497 - K03949 NDUFA5 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 5, at 1,418,367 to 1,418,907 12497
Group Condition mRNA_4125 mRNA_4126 mRNA_4127 mRNA_4128 mRNA_4129
12493 12494 12495 12496 12497
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.4 N.D. -1.5 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -1.0 -0.4 N.D. +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. -0.7 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 -0.0 N.D. +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.6 -0.1 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.1 N.D. -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.2 N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid -0.6 +0.3 N.D. -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.2 N.D. -0.1 N.D.
growth on oleic acid YNB Glucose -0.1 -0.0 N.D. -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.2 N.D. -0.1 N.D.
auxotrophy YNB Glucose -0.3 +0.3 N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.1 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.3 -0.0 N.D. +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.2 N.D. +0.1 N.D.
auxotrophy Dropout Complete +0.1 +0.3 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 N.D. +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.0 N.D. +0.1 N.D.
growth on ypd YPD +0.1 +0.3 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.2 N.D. +0.6 N.D.
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