Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12494 and 12495 are separated by 660 nucleotides12495 and 12496 are separated by 64 nucleotides12496 and 12497 are separated by 796 nucleotides12497 and 12498 are separated by 265 nucleotides mRNA_4126: 12494 - K20408 WDR24 WD repeat-containing protein 24, at 1,410,294 to 1,414,666 12494 mRNA_4127: 12495 - K00793 ribE, RIB5 riboflavin synthase, at 1,415,327 to 1,416,353 12495 mRNA_4128: 12496 - K02926 RP-L4, MRPL4, rplD large subunit ribosomal protein L4, at 1,416,418 to 1,417,570 12496 mRNA_4129: 12497 - K03949 NDUFA5 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 5, at 1,418,367 to 1,418,907 12497 mRNA_4130: 12498 - K14812 NGL2 RNA exonuclease NGL2, at 1,419,173 to 1,421,594 12498
Group Condition mRNA_4126 mRNA_4127 mRNA_4128 mRNA_4129 mRNA_4130
12494 12495 12496 12497 12498
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. -1.5 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.7 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.3 N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. +0.2 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. -0.1 N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. +0.0 N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. +0.1 N.D. -0.0
growth on oleic acid YNB Glucose -0.0 N.D. -0.1 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.1 N.D. -0.1
auxotrophy Dropout Complete +0.3 N.D. -0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. +0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.1 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.2 N.D. +0.3
growth on oleic acid YNB Oleic Acid +0.3 N.D. -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.1 N.D. -0.0
growth on ypd YPD +0.3 N.D. +0.2 N.D. -0.2
auxotrophy YNB Glucose +0.3 N.D. +0.1 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.6 N.D. -0.3
growth on ricinoleic acid YNB Glucose -0.0 N.D. +0.5 N.D. -0.0
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