Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12495 and 12496 are separated by 64 nucleotides12496 and 12497 are separated by 796 nucleotides12497 and 12498 are separated by 265 nucleotides12498 and 12499 are separated by 235 nucleotides mRNA_4127: 12495 - K00793 ribE, RIB5 riboflavin synthase, at 1,415,327 to 1,416,353 12495 mRNA_4128: 12496 - K02926 RP-L4, MRPL4, rplD large subunit ribosomal protein L4, at 1,416,418 to 1,417,570 12496 mRNA_4129: 12497 - K03949 NDUFA5 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 5, at 1,418,367 to 1,418,907 12497 mRNA_4130: 12498 - K14812 NGL2 RNA exonuclease NGL2, at 1,419,173 to 1,421,594 12498 mRNA_4131: 12499 - KOG3783 Uncharacterized conserved protein, at 1,421,830 to 1,424,236 12499
Group Condition mRNA_4127 mRNA_4128 mRNA_4129 mRNA_4130 mRNA_4131
12495 12496 12497 12498 12499
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.5 N.D. +0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.3 N.D. -0.3 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.7 N.D. -0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 N.D. +0.0 -0.5
auxotrophy Dropout Complete N.D. -0.2 N.D. -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 N.D. -0.1 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 N.D. -0.2 -0.2
growth on oleic acid YNB Glucose N.D. -0.1 N.D. -0.0 -0.1
auxotrophy YNB Glucose N.D. +0.1 N.D. +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. -0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. -0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 N.D. -0.3 +0.1
growth on ypd YPD N.D. +0.2 N.D. -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 N.D. -0.3 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. +0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 N.D. -0.3 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.3 +0.0
growth on ricinoleic acid YNB Glucose N.D. +0.5 N.D. -0.0 +0.2
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