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Protein
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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12513 and 12514 are separated by 896 nucleotides
12514 and 12515 are separated by 443 nucleotides
12515 and 12516 are separated by 194 nucleotides
12516 and 12517 are separated by 412 nucleotides
mRNA_4145: 12513 - K00641 metX homoserine O-acetyltransferase, at 1,460,929 to 1,462,648
12513
mRNA_4146: 12514 - Hypothetical Protein, at 1,463,545 to 1,465,730
12514
mRNA_4147: 12515 - HMMPfam-NAD(P)-binding Rossmann-like domain-PF13450,PRINTS-Adrenodoxin reductase family signature-PR00419,SUPERFAMILY--SSF51905, at 1,466,174 to 1,468,597
12515
mRNA_4148: 12516 - K00685 ATE1, ate1 arginine-tRNA-protein transferase, at 1,468,792 to 1,470,729
12516
mRNA_4149: 12517 - Hypothetical Protein, at 1,471,142 to 1,472,834
12517
Group
Condition
mRNA
_4145
mRNA
_4146
mRNA
_4147
mRNA
_4148
mRNA
_4149
12513
12514
12515
12516
12517
auxotrophy
YNB Glucose 75mM Arginine
-7.0
-0.4
-0.8
-0.4
+0.4
growth on ricinoleic acid
YNB Methylricinoleic Acid
-5.5
-0.4
+0.1
-0.4
+0.4
auxotrophy
YNB Glucose
-5.2
-0.2
-0.4
-0.5
+0.4
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-5.7
-0.1
+0.2
-0.5
+0.5
facs on lipid accumulation
Sorted No BODIPY Gate
-3.5
+0.0
-1.2
-0.2
-0.6
growth on oleic acid
YNB Glucose
-5.5
+0.3
-0.4
-0.1
+0.2
growth on ricinoleic acid
YNB Glucose
-5.4
-0.1
-0.1
-0.1
+0.4
facs on lipid accumulation
Sorted High BODIPY Gate
-3.0
-0.5
-1.2
+0.2
-0.6
buoyancy on lipid accumulation
40 hrs High Buoyancy
-5.0
-0.2
+0.0
-0.3
+0.5
growth on ricinoleic acid
YNB Ricinoleic Acid
-4.2
-0.7
+0.2
-0.4
+0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-5.2
-0.1
-0.5
+0.5
+0.8
growth on oleic acid
YNB Oleic Acid
-3.9
-0.1
-0.7
+0.0
+0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
-3.5
-0.2
-0.2
-0.0
-0.5
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-2.8
-0.3
+0.3
-0.2
+0.6
auxotrophy
YNB Glucose 75mM Methionine
-0.8
-0.0
-0.5
-0.2
+0.2
ypd buoyancy
40 hrs No Buoyancy Separation
+0.7
+0.1
-1.4
-0.3
-0.3
auxotrophy
Dropout Complete
-0.4
-0.3
-0.4
-0.4
+0.3
ypd buoyancy
40 hrs High Buoyancy
-0.2
+0.2
-0.3
+0.1
-0.4
ypd buoyancy
40 hrs Median Buoyancy
-0.1
+0.2
-0.4
+0.1
+0.1
growth on ypd
YPD
-0.3
+0.2
-0.5
+0.4
+0.3
ypd buoyancy
40 hrs Low Buoyancy
-0.0
+0.1
+0.5
+0.1
+0.1
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