Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12520 and 12521 are separated by 829 nucleotides12521 and 12522 are separated by 399 nucleotides12522 and 12523 are separated by 270 nucleotides12523 and 12524 are separated by 161 nucleotides mRNA_4152: 12520 - K15026 EIF2A translation initiation factor 2A, at 1,478,311 to 1,480,762 12520 mRNA_4153: 12521 - K20347 TMED2, EMP24 p24 family protein beta-1, at 1,481,592 to 1,482,556 12521 mRNA_4154: 12522 - K17402 MRPS23 small subunit ribosomal protein S23, at 1,482,956 to 1,484,034 12522 mRNA_4155: 12523 - K12492 ARFGAP1 ADP-ribosylation factor GTPase-activating protein 1, at 1,484,305 to 1,486,151 12523 mRNA_4156: 12524 - KOG1242 Protein containing adaptin N-terminal region, at 1,486,313 to 1,494,426 12524
Group Condition mRNA_4152 mRNA_4153 mRNA_4154 mRNA_4155 mRNA_4156
12520 12521 12522 12523 12524
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.4 N.D. -0.5 -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. -0.6 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 N.D. +0.3 -0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.3 N.D. -0.2 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.9 N.D. +0.1 -1.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -1.3 N.D. +0.3 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.9 N.D. +0.0 -1.4
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. +0.3 -1.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. -0.2 -0.1
auxotrophy YNB Glucose N.D. +0.3 N.D. +0.3 -0.9
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 N.D. -0.4 -0.1
growth on oleic acid YNB Glucose N.D. +0.2 N.D. +0.4 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. +0.0 +0.2
growth on oleic acid YNB Oleic Acid N.D. +0.5 N.D. +0.3 -0.8
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 N.D. +0.1 -0.5
growth on ypd YPD N.D. +0.2 N.D. -0.1 -0.0
auxotrophy Dropout Complete N.D. +0.7 N.D. -0.0 -0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. +1.0 N.D. -0.4 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 N.D. +0.3 +0.0
growth on ricinoleic acid YNB Glucose N.D. +0.9 N.D. +0.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.5 N.D. +0.3 +0.9
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