Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12527 and 12528 are separated by 184 nucleotides12528 and 12529 are separated by 299 nucleotides12529 and 12530 are separated by 347 nucleotides12530 and 12531 are separated by 508 nucleotides mRNA_4159: 12527 - K03002 RPA2, POLR1B DNA-directed RNA polymerase I subunit RPA2, at 1,498,210 to 1,502,276 12527 mRNA_4160: 12528 - Hypothetical Protein, at 1,502,461 to 1,503,563 12528 mRNA_4161: 12529 - SUPERFAMILY--SSF52833, at 1,503,863 to 1,504,762 12529 mRNA_4162: 12530 - Hypothetical Protein, at 1,505,110 to 1,508,834 12530 mRNA_4163: 12531 - Hypothetical Protein, at 1,509,343 to 1,510,830 12531
Group Condition mRNA_4159 mRNA_4160 mRNA_4161 mRNA_4162 mRNA_4163
12527 12528 12529 12530 12531
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.3 -0.9 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.4 -0.4 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.3 -0.3 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.3 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 -0.1 -0.4
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.0 -0.4 +0.0
auxotrophy YNB Glucose N.D. N.D. -0.1 -0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 +0.3 -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.2 -0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.2 +0.0 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.1 +0.0
growth on oleic acid YNB Glucose N.D. N.D. -0.1 -0.2 +0.1
growth on ypd YPD N.D. N.D. -0.3 +0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.0 -0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 -0.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 -0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.0 -0.2 +0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 +0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.1 +0.0 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.1 +0.3 -0.1
auxotrophy Dropout Complete N.D. N.D. +0.0 +0.1 +0.0
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