Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12534 and 12535 are separated by 516 nucleotides12535 and 12536 are separated by 411 nucleotides12536 and 12537 are separated by 647 nucleotides12537 and 12538 are separated by 1245 nucleotides mRNA_4166: 12534 - K12597 AIR1_2 protein AIR1/2, at 1,515,878 to 1,517,607 12534 mRNA_4167: 12535 - Hypothetical Protein, at 1,518,124 to 1,518,875 12535 mRNA_4168: 12536 - K09487 HSP90B, TRA1 heat shock protein 90kDa beta, at 1,519,287 to 1,521,954 12536 mRNA_4169: 12537 - K13299 GSTK1 glutathione S-transferase kappa 1, at 1,522,602 to 1,523,728 12537 mRNA_4170: 12538 - KOG1256 Long-chain acyl-CoA synthetases (AMP-forming), at 1,524,974 to 1,527,080 12538
Group Condition mRNA_4166 mRNA_4167 mRNA_4168 mRNA_4169 mRNA_4170
12534 12535 12536 12537 12538
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 -0.3 -0.1 +0.2 -0.3
growth on oleic acid YNB Glucose -0.0 +0.0 -0.0 -0.7 -0.1
growth on ypd YPD -0.4 -0.2 +0.3 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 -0.1 +0.0 +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 -0.1 +0.1 +0.3 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.2 -0.0 +0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.3 +0.0 +0.1 +0.1
auxotrophy Dropout Complete +0.0 -0.4 +0.1 -0.0 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.3 +0.1 -0.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.5 -0.4 +0.0 +0.7 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.1 +0.0 -0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.3 +0.0 -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.0 +0.1 -0.3 +0.0
growth on ricinoleic acid YNB Glucose +0.1 -0.2 +0.1 -0.1 +0.0
auxotrophy YNB Glucose +0.2 -0.1 +0.1 -0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.0 +0.1 -0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.0 +0.4 -0.3 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.0 +0.1 -0.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.2 +0.3 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.0 +0.3 +0.1 -0.1
growth on oleic acid YNB Oleic Acid +0.2 +0.4 +0.2 -0.2 +0.1
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