Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12538 and 12539 are separated by 933 nucleotides12539 and 12540 are separated by 561 nucleotides12540 and 12541 are separated by 1700 nucleotides12541 and 12542 are separated by 1110 nucleotides mRNA_4170: 12538 - KOG1256 Long-chain acyl-CoA synthetases (AMP-forming), at 1,524,974 to 1,527,080 12538 mRNA_4171: 12539 - KOG2806 Chitinase, at 1,528,014 to 1,529,581 12539 mRNA_4172: 12540 - KOG1212 Amidases, at 1,530,143 to 1,531,631 12540 mRNA_4173: 12541 - KOG2348 Urea transporter, at 1,533,332 to 1,534,654 12541 mRNA_4174: 12542 - KOG4305 RhoGEF GTPase, at 1,535,765 to 1,540,719 12542
Group Condition mRNA_4170 mRNA_4171 mRNA_4172 mRNA_4173 mRNA_4174
12538 12539 12540 12541 12542
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 -0.6 N.D. -0.4 -1.1
auxotrophy YNB Glucose +0.1 -0.6 N.D. -0.4 -0.5
growth on ricinoleic acid YNB Glucose +0.0 -0.6 N.D. -0.3 -0.6
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.4 N.D. -0.7 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.5 N.D. -0.6 -0.2
growth on oleic acid YNB Glucose -0.1 -0.1 N.D. -0.5 -0.4
auxotrophy Dropout Complete +0.0 -0.5 N.D. -0.4 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.5 N.D. -0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.1 N.D. -0.2 -0.6
growth on oleic acid YNB Oleic Acid +0.1 -0.1 N.D. -0.7 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.1 N.D. -0.1 +0.1
growth on ypd YPD +0.1 -0.2 N.D. +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.4 N.D. +0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.1 N.D. +0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.2 N.D. +0.0 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.2 N.D. +0.5 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.4 N.D. -0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.1 N.D. -0.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.9 N.D. -0.2 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.7 N.D. -0.0 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.6 N.D. +0.4 -0.3
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