Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12539 and 12540 are separated by 561 nucleotides12540 and 12541 are separated by 1700 nucleotides12541 and 12542 are separated by 1110 nucleotides12542 and 12543 are separated by 486 nucleotides mRNA_4171: 12539 - KOG2806 Chitinase, at 1,528,014 to 1,529,581 12539 mRNA_4172: 12540 - KOG1212 Amidases, at 1,530,143 to 1,531,631 12540 mRNA_4173: 12541 - KOG2348 Urea transporter, at 1,533,332 to 1,534,654 12541 mRNA_4174: 12542 - KOG4305 RhoGEF GTPase, at 1,535,765 to 1,540,719 12542 mRNA_4175: 12543 - K03939 NDUFS6 NADH dehydrogenase (ubiquinone) Fe-S protein 6, at 1,541,206 to 1,541,936 12543
Group Condition mRNA_4171 mRNA_4172 mRNA_4173 mRNA_4174 mRNA_4175
12539 12540 12541 12542 12543
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 N.D. -0.4 -1.1 -2.8
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. -0.2 -0.6 -2.9
growth on ricinoleic acid YNB Glucose -0.6 N.D. -0.3 -0.6 -1.0
ypd buoyancy 40 hrs Low Buoyancy -0.5 N.D. -0.1 -0.1 -1.3
auxotrophy YNB Glucose -0.6 N.D. -0.4 -0.5 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.5 N.D. -0.6 -0.2 -0.2
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.7 +0.1 -0.8
growth on oleic acid YNB Glucose -0.1 N.D. -0.5 -0.4 -0.3
auxotrophy Dropout Complete -0.5 N.D. -0.4 -0.0 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.2 -0.2 -1.3
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. -0.7 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.1 +0.1 -0.3
growth on ypd YPD -0.2 N.D. +0.1 -0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.0 +0.2 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.9 N.D. -0.2 -0.4 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -0.1 +0.3 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. +0.5 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. -0.0 -0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. +0.0 +0.2 +0.6
facs on lipid accumulation Sorted No BODIPY Gate +0.7 N.D. -0.0 -0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 N.D. +0.4 -0.3 +0.7
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