Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12542 and 12543 are separated by 486 nucleotides12543 and 12544 are separated by 104 nucleotides12544 and 12545 are separated by 272 nucleotides12545 and 12546 are separated by 165 nucleotides mRNA_4174: 12542 - KOG4305 RhoGEF GTPase, at 1,535,765 to 1,540,719 12542 mRNA_4175: 12543 - K03939 NDUFS6 NADH dehydrogenase (ubiquinone) Fe-S protein 6, at 1,541,206 to 1,541,936 12543 mRNA_4176: 12544 - KOG4093 Uncharacterized conserved protein, at 1,542,041 to 1,542,823 12544 mRNA_4177: 12545 - K15356 VRG4, GONST1 GDP-mannose transporter, at 1,543,096 to 1,544,844 12545 mRNA_4178: 12546 - KOG1337 N-methyltransferase, at 1,545,010 to 1,546,616 12546
Group Condition mRNA_4174 mRNA_4175 mRNA_4176 mRNA_4177 mRNA_4178
12542 12543 12544 12545 12546
growth on ricinoleic acid YNB Methylricinoleic Acid -1.1 -2.8 N.D. N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -2.9 N.D. N.D. -0.4
growth on ricinoleic acid YNB Glucose -0.6 -1.0 N.D. N.D. -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.1 -1.3 N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -1.3 N.D. N.D. -0.0
auxotrophy YNB Glucose -0.5 -0.3 N.D. N.D. -0.2
growth on oleic acid YNB Glucose -0.4 -0.3 N.D. N.D. +0.0
growth on oleic acid YNB Oleic Acid +0.1 -0.8 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.3 N.D. N.D. -0.3
growth on ypd YPD -0.3 -0.1 N.D. N.D. -0.2
auxotrophy Dropout Complete -0.0 -0.3 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.2 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.0 N.D. N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.1 N.D. N.D. +0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.1 N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.0 N.D. N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.7 N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.1 N.D. N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.1 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.6 N.D. N.D. -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.0 N.D. N.D. +0.3
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