Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12543 and 12544 are separated by 104 nucleotides12544 and 12545 are separated by 272 nucleotides12545 and 12546 are separated by 165 nucleotides12546 and 12547 are separated by 721 nucleotides mRNA_4175: 12543 - K03939 NDUFS6 NADH dehydrogenase (ubiquinone) Fe-S protein 6, at 1,541,206 to 1,541,936 12543 mRNA_4176: 12544 - KOG4093 Uncharacterized conserved protein, at 1,542,041 to 1,542,823 12544 mRNA_4177: 12545 - K15356 VRG4, GONST1 GDP-mannose transporter, at 1,543,096 to 1,544,844 12545 mRNA_4178: 12546 - KOG1337 N-methyltransferase, at 1,545,010 to 1,546,616 12546 mRNA_4179: 12547 - KOG0429 Ubiquitin-conjugating enzyme-related protein Ft1, involved in programmed cell death, at 1,547,338 to 1,548,132 12547
Group Condition mRNA_4175 mRNA_4176 mRNA_4177 mRNA_4178 mRNA_4179
12543 12544 12545 12546 12547
growth on ricinoleic acid YNB Methylricinoleic Acid -2.8 N.D. N.D. -0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -2.9 N.D. N.D. -0.4 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. N.D. -0.3 -1.8
ypd buoyancy 40 hrs Low Buoyancy -1.3 N.D. N.D. -0.5 -0.1
growth on ricinoleic acid YNB Glucose -1.0 N.D. N.D. -0.5 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.3 N.D. N.D. -0.0 +0.2
auxotrophy Dropout Complete -0.3 N.D. N.D. -0.1 -0.3
auxotrophy YNB Glucose -0.3 N.D. N.D. -0.2 -0.3
growth on oleic acid YNB Oleic Acid -0.8 N.D. N.D. -0.1 +0.1
growth on ypd YPD -0.1 N.D. N.D. -0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. N.D. -0.0 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. N.D. -0.5 -0.7
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. +0.1 -0.5
growth on oleic acid YNB Glucose -0.3 N.D. N.D. +0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. N.D. +0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. N.D. +0.3 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. -0.2 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.2 +0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. +0.0 +0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. N.D. +0.5 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 N.D. N.D. -0.3 +0.7
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