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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12548 and 12549 are separated by 337 nucleotides
12549 and 12550 are separated by 772 nucleotides
12550 and 12551 are separated by 150 nucleotides
12551 and 12552 are separated by 527 nucleotides
mRNA_4180: 12548 - K03260 EIF4G translation initiation factor 4G, at 1,548,392 to 1,550,884
12548
mRNA_4181: 12549 - Hypothetical Protein, at 1,551,222 to 1,553,388
12549
mRNA_4182: 12550 - K13288 orn, REX2, REXO2 oligoribonuclease, at 1,554,161 to 1,555,375
12550
mRNA_4183: 12551 - K01497 ribA, RIB1 GTP cyclohydrolase II, at 1,555,526 to 1,557,851
12551
mRNA_4184: 12552 - K20989 DUR3 urea-proton symporter, at 1,558,379 to 1,561,248
12552
Group
Condition
mRNA
_4180
mRNA
_4181
mRNA
_4182
mRNA
_4183
mRNA
_4184
12548
12549
12550
12551
12552
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
N.D.
-0.7
N.D.
-0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
N.D.
-0.7
N.D.
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
N.D.
-0.6
N.D.
-0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
N.D.
-0.3
N.D.
-0.3
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
N.D.
+0.2
N.D.
-0.7
growth on oleic acid
YNB Oleic Acid
N.D.
N.D.
-0.2
N.D.
-0.3
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
N.D.
-0.2
N.D.
-0.2
growth on oleic acid
YNB Glucose
N.D.
N.D.
+0.0
N.D.
-0.3
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
N.D.
-0.2
N.D.
-0.0
growth on ricinoleic acid
YNB Glucose
N.D.
N.D.
-0.0
N.D.
-0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
N.D.
-0.1
N.D.
-0.0
growth on ypd
YPD
N.D.
N.D.
+0.1
N.D.
-0.2
auxotrophy
Dropout Complete
N.D.
N.D.
+0.4
N.D.
-0.3
auxotrophy
YNB Glucose 75mM Arginine
N.D.
N.D.
+0.3
N.D.
-0.2
ypd buoyancy
40 hrs Low Buoyancy
N.D.
N.D.
+0.2
N.D.
-0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
N.D.
+0.1
N.D.
+0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
N.D.
+0.1
N.D.
+0.1
auxotrophy
YNB Glucose
N.D.
N.D.
+0.3
N.D.
-0.0
ypd buoyancy
40 hrs High Buoyancy
N.D.
N.D.
+0.4
N.D.
+0.1
auxotrophy
YNB Glucose 75mM Methionine
N.D.
N.D.
+0.6
N.D.
-0.1
ypd buoyancy
40 hrs Median Buoyancy
N.D.
N.D.
+0.7
N.D.
+0.2
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