Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12549 and 12550 are separated by 772 nucleotides12550 and 12551 are separated by 150 nucleotides12551 and 12552 are separated by 527 nucleotides12552 and 12553 are separated by 845 nucleotides mRNA_4181: 12549 - Hypothetical Protein, at 1,551,222 to 1,553,388 12549 mRNA_4182: 12550 - K13288 orn, REX2, REXO2 oligoribonuclease, at 1,554,161 to 1,555,375 12550 mRNA_4183: 12551 - K01497 ribA, RIB1 GTP cyclohydrolase II, at 1,555,526 to 1,557,851 12551 mRNA_4184: 12552 - K20989 DUR3 urea-proton symporter, at 1,558,379 to 1,561,248 12552 mRNA_4185: 12553 - KOG1212 Amidases, at 1,562,094 to 1,563,941 12553
Group Condition mRNA_4181 mRNA_4182 mRNA_4183 mRNA_4184 mRNA_4185
12549 12550 12551 12552 12553
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.7 N.D. -0.3 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.6 N.D. -0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.7 N.D. -0.1 +0.2
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. -0.3 +0.2
growth on oleic acid YNB Glucose N.D. +0.0 N.D. -0.3 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 N.D. -0.3 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 N.D. -0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 N.D. -0.2 -0.3
auxotrophy Dropout Complete N.D. +0.4 N.D. -0.3 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 N.D. -0.7 +0.4
growth on ypd YPD N.D. +0.1 N.D. -0.2 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 N.D. -0.2 +0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 N.D. -0.0 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.6 N.D. -0.1 -0.4
growth on ricinoleic acid YNB Glucose N.D. -0.0 N.D. -0.2 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 N.D. -0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 N.D. +0.1 +0.0
auxotrophy YNB Glucose N.D. +0.3 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 N.D. +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.4 N.D. +0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.7 N.D. +0.2 +0.3
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