Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12550 and 12551 are separated by 150 nucleotides12551 and 12552 are separated by 527 nucleotides12552 and 12553 are separated by 845 nucleotides12553 and 12554 are separated by 558 nucleotides mRNA_4182: 12550 - K13288 orn, REX2, REXO2 oligoribonuclease, at 1,554,161 to 1,555,375 12550 mRNA_4183: 12551 - K01497 ribA, RIB1 GTP cyclohydrolase II, at 1,555,526 to 1,557,851 12551 mRNA_4184: 12552 - K20989 DUR3 urea-proton symporter, at 1,558,379 to 1,561,248 12552 mRNA_4185: 12553 - KOG1212 Amidases, at 1,562,094 to 1,563,941 12553 mRNA_4186: 12554 - KOG2806 Chitinase, at 1,564,500 to 1,566,078 12554
Group Condition mRNA_4182 mRNA_4183 mRNA_4184 mRNA_4185 mRNA_4186
12550 12551 12552 12553 12554
facs on lipid accumulation Sorted High BODIPY Gate -0.6 N.D. -0.1 -0.0 -1.8
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 N.D. -0.1 +0.2 -1.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 N.D. -0.3 +0.3 -0.6
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.1 +0.0 -1.4
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.3 +0.2 -0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. -0.3 +0.3 -0.9
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.0 +0.3 -1.0
growth on oleic acid YNB Glucose +0.0 N.D. -0.3 -0.0 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -0.2 +0.4 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -0.7 +0.4 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. -0.0 -0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. +0.1 +0.2 -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.1 +0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.6 N.D. -0.1 -0.4 +0.1
auxotrophy Dropout Complete +0.4 N.D. -0.3 -0.2 +0.3
growth on ricinoleic acid YNB Glucose -0.0 N.D. -0.2 +0.4 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.2 -0.3 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. +0.1 +0.2 -0.1
auxotrophy YNB Glucose +0.3 N.D. -0.0 +0.1 +0.6
growth on ypd YPD +0.1 N.D. -0.2 +0.0 +1.2
ypd buoyancy 40 hrs Median Buoyancy +0.7 N.D. +0.2 +0.3 +0.2
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