Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12553 and 12554 are separated by 558 nucleotides12554 and 12555 are separated by 334 nucleotides12555 and 12556 are separated by 167 nucleotides12556 and 12557 are separated by 411 nucleotides mRNA_4185: 12553 - KOG1212 Amidases, at 1,562,094 to 1,563,941 12553 mRNA_4186: 12554 - KOG2806 Chitinase, at 1,564,500 to 1,566,078 12554 mRNA_4187: 12555 - K01897 ACSL, fadD long-chain acyl-CoA synthetase, at 1,566,413 to 1,568,898 12555 mRNA_4188: 12556 - KOG4372 Predicted alpha/beta hydrolase, at 1,569,066 to 1,571,020 12556 mRNA_4189: 12557 - Hypothetical Protein, at 1,571,432 to 1,574,972 12557
Group Condition mRNA_4185 mRNA_4186 mRNA_4187 mRNA_4188 mRNA_4189
12553 12554 12555 12556 12557
growth on oleic acid YNB Oleic Acid +0.2 -0.9 -1.5 -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -1.4 -0.0 -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -1.8 -0.3 +0.3 N.D.
growth on oleic acid YNB Glucose -0.0 -0.4 -1.1 -0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.1 -0.5 -0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -1.1 -0.7 +0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.6 -0.8 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.6 -0.4 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.1 +0.0 -0.7 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -1.0 +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.9 -0.2 +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.5 -0.6 -0.1 N.D.
auxotrophy Dropout Complete -0.2 +0.3 -0.4 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.0 -0.4 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.4 -0.4 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.5 +0.8 -0.8 N.D.
auxotrophy YNB Glucose +0.1 +0.6 -0.3 -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.1 +0.4 -0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.4 +0.2 -0.2 -0.1 N.D.
growth on ypd YPD +0.0 +1.2 -0.4 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.2 +0.3 +0.1 N.D.
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