Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12556 and 12557 are separated by 411 nucleotides12557 and 12558 are separated by 68 nucleotides12558 and 12559 are separated by 1045 nucleotides12559 and 12560 are separated by 208 nucleotides mRNA_4188: 12556 - KOG4372 Predicted alpha/beta hydrolase, at 1,569,066 to 1,571,020 12556 mRNA_4189: 12557 - Hypothetical Protein, at 1,571,432 to 1,574,972 12557 mRNA_4190: 12558 - Hypothetical Protein, at 1,575,041 to 1,575,964 12558 mRNA_4191: 12559 - HMMPfam-GAF domain-PF13492,SMART-Domain present in phytochromes and cGMP-specific phosphodiesterases.-SM00065,SUPERFAMILY--SSF55781, at 1,577,010 to 1,579,239 12559 mRNA_4192: 12560 - K04797 pfdA, PFDN5 prefoldin alpha subunit, at 1,579,448 to 1,580,118 12560
Group Condition mRNA_4188 mRNA_4189 mRNA_4190 mRNA_4191 mRNA_4192
12556 12557 12558 12559 12560
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. -0.7 -0.1 -2.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. -1.2 -0.9 +0.9
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.2 -0.2 -0.9
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. -1.0 +0.2 -0.2
auxotrophy YNB Glucose -0.3 N.D. +0.6 +0.0 -0.9
facs on lipid accumulation Sorted High BODIPY Gate +0.3 N.D. -1.1 +0.5 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.7 N.D. +0.3 -0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. -0.2 -0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 N.D. -0.1 -0.5 -0.3
growth on oleic acid YNB Oleic Acid -0.1 N.D. +0.5 -0.1 -0.2
growth on oleic acid YNB Glucose -0.4 N.D. +0.1 +0.2 +0.3
growth on ypd YPD -0.2 N.D. -0.3 +0.3 +0.5
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.4 -0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. +0.4 +0.5 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.0 -0.0 +0.3
auxotrophy Dropout Complete -0.2 N.D. +0.2 +0.3 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +1.0 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.3 +0.0 +1.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.8 +0.0 +0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. -0.2 -0.2 +2.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.1 +0.0 +2.3
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