Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12559 and 12560 are separated by 208 nucleotides12560 and 12561 are separated by 309 nucleotides12561 and 12562 are separated by 246 nucleotides12562 and 12563 are separated by 172 nucleotides mRNA_4191: 12559 - HMMPfam-GAF domain-PF13492,SMART-Domain present in phytochromes and cGMP-specific phosphodiesterases.-SM00065,SUPERFAMILY--SSF55781, at 1,577,010 to 1,579,239 12559 mRNA_4192: 12560 - K04797 pfdA, PFDN5 prefoldin alpha subunit, at 1,579,448 to 1,580,118 12560 mRNA_4193: 12561 - Hypothetical Protein, at 1,580,428 to 1,584,449 12561 mRNA_4194: 12562 - K01934 MTHFS 5-formyltetrahydrofolate cyclo-ligase, at 1,584,696 to 1,585,623 12562 mRNA_4195: 12563 - HMMPfam-Protein of unknown function (DUF2962)-PF11176, at 1,585,796 to 1,586,681 12563
Group Condition mRNA_4191 mRNA_4192 mRNA_4193 mRNA_4194 mRNA_4195
12559 12560 12561 12562 12563
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -2.0 -0.0 -0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.2 -0.9 -0.1 -0.0 N.D.
auxotrophy YNB Glucose +0.0 -0.9 -0.0 -0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 -0.3 -0.2 +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.2 -0.1 -0.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.2 -0.1 -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 +0.9 +0.5 -1.1 N.D.
growth on oleic acid YNB Oleic Acid -0.1 -0.2 -0.1 -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.2 +0.1 -0.6 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.3 -0.1 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.3 +0.1 -0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.2 +0.1 -0.3 N.D.
growth on oleic acid YNB Glucose +0.2 +0.3 -0.1 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.5 -0.2 +0.5 -0.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.2 +0.2 +0.2 N.D.
auxotrophy Dropout Complete +0.3 +0.3 -0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.9 +0.2 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +1.2 +0.1 +0.1 N.D.
growth on ypd YPD +0.3 +0.5 +0.1 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +2.1 +0.0 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +2.3 +0.0 +0.5 N.D.
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