Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12560 and 12561 are separated by 309 nucleotides12561 and 12562 are separated by 246 nucleotides12562 and 12563 are separated by 172 nucleotides12563 and 12564 are separated by 156 nucleotides mRNA_4192: 12560 - K04797 pfdA, PFDN5 prefoldin alpha subunit, at 1,579,448 to 1,580,118 12560 mRNA_4193: 12561 - Hypothetical Protein, at 1,580,428 to 1,584,449 12561 mRNA_4194: 12562 - K01934 MTHFS 5-formyltetrahydrofolate cyclo-ligase, at 1,584,696 to 1,585,623 12562 mRNA_4195: 12563 - HMMPfam-Protein of unknown function (DUF2962)-PF11176, at 1,585,796 to 1,586,681 12563 mRNA_4196: 12564 - K12343 SRD5A1 3-oxo-5-alpha-steroid 4-dehydrogenase 1, at 1,586,838 to 1,587,953 12564
Group Condition mRNA_4192 mRNA_4193 mRNA_4194 mRNA_4195 mRNA_4196
12560 12561 12562 12563 12564
facs on lipid accumulation Sorted Low BODIPY Gate -2.0 -0.0 -0.2 N.D. +0.1
auxotrophy YNB Glucose -0.9 -0.0 -0.3 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.1 -0.6 N.D. -0.5
growth on ricinoleic acid YNB Glucose -0.9 -0.1 -0.0 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.1 -0.6 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.1 -0.2 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.2 +0.0 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 -0.3 N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.5 -0.6 N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.9 +0.5 -1.1 N.D. -0.7
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.1 -0.5 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.2 +0.2 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.1 -0.1 N.D. -0.2
growth on oleic acid YNB Glucose +0.3 -0.1 -0.2 N.D. +0.0
growth on oleic acid YNB Oleic Acid -0.2 -0.1 -0.2 N.D. +0.6
auxotrophy Dropout Complete +0.3 -0.1 -0.1 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.2 +0.1 +0.1 N.D. -0.2
growth on ypd YPD +0.5 +0.1 +0.5 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.9 +0.2 +0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +2.1 +0.0 +0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +2.3 +0.0 +0.5 N.D. -0.2
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