Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12561 and 12562 are separated by 246 nucleotides12562 and 12563 are separated by 172 nucleotides12563 and 12564 are separated by 156 nucleotides12564 and 12565 are separated by 378 nucleotides mRNA_4193: 12561 - Hypothetical Protein, at 1,580,428 to 1,584,449 12561 mRNA_4194: 12562 - K01934 MTHFS 5-formyltetrahydrofolate cyclo-ligase, at 1,584,696 to 1,585,623 12562 mRNA_4195: 12563 - HMMPfam-Protein of unknown function (DUF2962)-PF11176, at 1,585,796 to 1,586,681 12563 mRNA_4196: 12564 - K12343 SRD5A1 3-oxo-5-alpha-steroid 4-dehydrogenase 1, at 1,586,838 to 1,587,953 12564 mRNA_4197: 12565 - K00512 CYP17A steroid 17alpha-monooxygenase / 17alpha-hydroxyprogesterone deacetylase, at 1,588,332 to 1,590,437 12565
Group Condition mRNA_4193 mRNA_4194 mRNA_4195 mRNA_4196 mRNA_4197
12561 12562 12563 12564 12565
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.6 N.D. -0.5 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.5 -0.6 N.D. -0.4 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.2 N.D. -0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.3 N.D. -0.4 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 N.D. -0.2 -0.7
auxotrophy YNB Glucose -0.0 -0.3 N.D. -0.3 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.6 N.D. -0.3 +0.4
growth on oleic acid YNB Glucose -0.1 -0.2 N.D. +0.0 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 -1.1 N.D. -0.7 +0.8
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.0 N.D. -0.2 +0.1
growth on ricinoleic acid YNB Glucose -0.1 -0.0 N.D. +0.1 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.2 N.D. -0.3 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.2 N.D. +0.1 +0.1
auxotrophy Dropout Complete -0.1 -0.1 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.3 N.D. -0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.5 N.D. -0.2 +0.8
growth on oleic acid YNB Oleic Acid -0.1 -0.2 N.D. +0.6 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.5 N.D. -0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.1 N.D. -0.2 +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.1 N.D. +0.2 -0.1
growth on ypd YPD +0.1 +0.5 N.D. +0.2 -0.1
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